循环 miR-338-5p 是结直肠癌的潜在诊断生物标志物。

Circulating miR-338-5p is a potential diagnostic biomarker in colorectal cancer.

机构信息

Department of Gastroenterology, Zhongshan Hospital of Fudan University, Shanghai, China.

Shanghai Institute of Liver Diseases, Zhongshan Hospital of Fudan University, Shanghai, China.

出版信息

J Dig Dis. 2018 Jul;19(7):404-410. doi: 10.1111/1751-2980.12643.

DOI:10.1111/1751-2980.12643

PMID:29952077

Abstract

OBJECTIVE

The expression of miR-338-5p has been reported to be upregulated in colorectal cancer (CRC) tissues. Clinicopathological features indicate that miR-338-5p overexpression correlates with the metastatic status of CRC. This study was aimed to investigate the diagnostic value of serum miR-338-5p for CRC.

METHODS

Peripheral blood samples were collected from 210 participants, including 80 patients with CRC, 50 with colorectal polyps and 80 healthy controls. Serum miR-338-5p was quantified by quantitative reverse-transcription polymerase chain reaction. The area under the receiver operating characteristic curve (AUROC) was used to estimate the diagnostic value of miR-338-5p, carcinoembryonic antigen (CEA) and the combination of these two biomarkers.

RESULTS

Serum miR-338-5p levels (fold change) in patients with CRC and colorectal polyps, and controls were 4.94 ± 1.13, 4.12 ± 0.75 and 3.07 ± 0.75, respectively. Significant differences were observed between the groups (P < 0.001). The AUROC of miR-338-5p was 0.923 (95% CI 0.882-0.964) and 0.845 (95% CI 0.792-0.898), respectively, for distinguishing CRC from healthy controls or from those without CRC. The AUROC of the combination of miR-338-5p and CEA was 0.932 (95% CI 0.882-0.964), with a sensitivity of 85%, a specificity of 88.8% at a cut-off value of 8.16.

CONCLUSIONS

Circulating miR-338-5p may serve as a potential noninvasive diagnostic biomarker for detecting CRC. The combination of miR-338-5p and CEA exhibits the highest diagnostic value in our study.

摘要

目的

已有研究报道 miR-338-5p 在结直肠癌（CRC）组织中呈高表达。临床病理特征表明，miR-338-5p 过表达与 CRC 的转移状态相关。本研究旨在探讨血清 miR-338-5p 对 CRC 的诊断价值。

方法

收集 210 名参与者的外周血样本，包括 80 例 CRC 患者、50 例结直肠息肉患者和 80 例健康对照者。采用实时定量逆转录聚合酶链反应（qRT-PCR）定量检测血清 miR-338-5p。采用受试者工作特征曲线（ROC）下面积（AUC）评估 miR-338-5p、癌胚抗原（CEA）及两者联合检测对 CRC 的诊断价值。

结果

CRC 患者、结直肠息肉患者和健康对照者血清 miR-338-5p 水平（倍数变化）分别为 4.94±1.13、4.12±0.75 和 3.07±0.75，组间比较差异有统计学意义（P<0.001）。miR-338-5p 鉴别 CRC 与健康对照者和无 CRC 者的 AUC 分别为 0.923（95%CI 0.882~~0.964）和 0.845（95%CI 0.792~~0.898）。miR-338-5p 和 CEA 联合检测的 AUC 为 0.932（95%CI 0.882~0.964），其最佳截断值为 8.16，灵敏度为 85%，特异度为 88.8%。