miR-143-3p 和 -424-5p 在结直肠癌中的表达水平及其临床意义。

Expression levels of miR-143-3p and -424-5p in colorectal cancer and their clinical significance.

机构信息

Department of Medical Genetics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

出版信息

Cancer Biomark. 2019;24(3):291-297. doi: 10.3233/CBM-182171.

DOI:10.3233/CBM-182171

PMID:30883340

Abstract

BACKGROUND

Colorectal cancer (CRC) is one of the most prevalent cancers and microRNAs are involved in colorectal carcinogenesis and progression. The role of our candidate microRNAs (miR-143-3p, -424-5p, -212-3p and -34a-3p) have been investigated in various cancers.

OBJECTIVE

The aim of the current study was to evaluate expression levels of microRNAs (miR-143-3p, -424-5p, -212-3p and -34a-3p) in the sera of patients with CRC in order to identify potential non-invasive biomarker for CRC and investigate the relationship between their expression and clinicopathological features of CRC.

METHODS

The serological expression of candidate microRNAs were measured in 124 participants, including 62 CRC patients and 62 healthy controls and the serum expression levels of candidate miRNAs were quantified by stemloop reverse transcriptionquantitative polymerase chain reaction.

RESULTS

In the present study, results showed a significant upregulation expression level of miR-424-5p (P< 0.001) and decreased expression level of miR143-3p was observed in the sera of patients with CRC (P< 0.001). Receiver operating characteristic (ROC) curve analysis demonstrated the area of miR-424-5p and miR-143-3p under the ROC curve for CRC diagnosis were 0.703 and 0.724 respectively (P< 0.001). In addition, down expression of miR-143-3p was significantly associated with tumor size (P= 0.005) and lymph node metastasis (P= 0.020) in CRC patients.

CONCLUSIONS

The present investigation suggested that low expression of miR-143-3p and increasing level of miR-424-5p in CRC patients may play an important role in development of CRC and they could function as potential non-invasive biomarkers for CRC.

摘要

背景

结直肠癌（CRC）是最常见的癌症之一，microRNAs 参与了结直肠癌的发生和发展。我们候选 microRNAs（miR-143-3p、-424-5p、-212-3p 和 -34a-3p）在各种癌症中的作用已经被研究过。

目的

本研究旨在评估 microRNAs（miR-143-3p、-424-5p、-212-3p 和 -34a-3p）在 CRC 患者血清中的表达水平，以确定 CRC 的潜在非侵入性生物标志物，并研究其表达与 CRC 的临床病理特征之间的关系。

方法

采用茎环逆转录定量聚合酶链反应（stemloop RT-qPCR）检测 124 名参与者（包括 62 名 CRC 患者和 62 名健康对照者）候选 microRNAs 的血清表达水平。

结果

本研究结果显示，CRC 患者血清中 miR-424-5p 的表达水平显著上调（P<0.001），miR143-3p 的表达水平下调（P<0.001）。受试者工作特征（ROC）曲线分析表明，miR-424-5p 和 miR-143-3p 在 ROC 曲线下的 CRC 诊断面积分别为 0.703 和 0.724（P<0.001）。此外，miR-143-3p 的低表达与 CRC 患者的肿瘤大小（P=0.005）和淋巴结转移（P=0.020）显著相关。