臭椿抗烟草花叶病毒查尔酮类化合物。

Anti-Tobacco Mosaic Virus Quassinoids from Ailanthus altissima (Mill.) Swingle.

出版信息

J Agric Food Chem. 2018 Jul 18;66(28):7347-7357. doi: 10.1021/acs.jafc.8b01280. Epub 2018 Jul 10.

DOI:10.1021/acs.jafc.8b01280

Abstract

Quassinoids are bitter constituents characteristic of the family Simaroubaceae. A total of 18 C quassinoids, including nine new quassinoid glycosides, named chuglycosides A-I (1-6 and 8-10), were identified from the samara of Ailanthus altissima (Mill.) Swingle. All of the quassinoids showed potent anti-tobacco mosaic virus (TMV) activity. A preliminary structure-anti-TMV activity relationship of quassinoids was discussed. The effects of three quassinoids, including chaparrinone (12), glaucarubinone (15), and ailanthone (16), on the accumulation of TMV coat protein (CP) were studied by western blot analysis. Ailanthone (16) was further investigated for its influence on TMV spread in the Nicotiana benthamiana plant.

摘要

苦木苦味素是苦木科植物的特征性成分。从臭椿的翅果中分离得到 18 个 C 型苦木苦味素，包括 9 个新的苦木苦味素糖苷，命名为 chuglycosides A-I（1-6 和 8-10）。所有苦木苦味素均表现出较强的抗烟草花叶病毒（TMV）活性。讨论了苦木苦味素的结构-抗 TMV 活性关系。通过 Western blot 分析研究了三种苦木苦味素，包括 chaparrinone（12）、glaucarubinone（15）和 ailan-thone（16）对 TMV 外壳蛋白（CP）积累的影响。进一步研究了 ailan-thone（16）对烟草原生质体中 TMV 传播的影响。