Koganti Praveen, Tulsawani Rajkumar, Sharma Purva, Sharma Manish, Arora Shivani, Misra Kshipra
Defence Institute of Physiology and Allied Sciences, Timarpur, Delhi, India.
Defence Institute of Physiology and Allied Sciences, DRDO, Timarpur, Delhi, India.
Int J Med Mushrooms. 2018;20(5):431-444. doi: 10.1615/IntJMedMushrooms.2018026075.
Ganoderma lucidum is known to exert many health benefits including effects to improve oxygen utilization. Therefore, this study was designed to evaluate the role of a hydroalcoholic G. lucidum extract in providing tolerance to HT22 cells grown under hypoxic conditions. HT22 cells were exposed to 0.5% O2 in the presence or absence of the extract for 24 hours. At the end of the exposure period, we performed cell viability assays, cell cycle analysis, and biochemical and protein expression studies. The extract-treated cells revealed less cell death, minimized caspase 3 and reactive oxygen species levels, and relieved G0/G1 cell cycle arrest compared with hypoxic cells cultured without the extract. Further, extract-treated cells showed improved expression of Nrf2, heme oxygenase 1, and metallothionein and stabilized levels of hypoxia-inducible factor 1α. Moreover, lower levels of nuclear factor-κB and tumor necrosis factor a were evident in extract-treated cells. Overall, the G. lucidum extract reduced hypoxia-induced cell death and augmented transcription factors (HIF-1α and Nrf2), conferring tolerance to hypoxia.
众所周知，灵芝具有诸多健康益处，包括改善氧气利用的作用。因此，本研究旨在评估灵芝水醇提取物在为缺氧条件下生长的HT22细胞提供耐受性方面的作用。将HT22细胞在有或无提取物的情况下暴露于0.5%的氧气中24小时。在暴露期结束时，我们进行了细胞活力测定、细胞周期分析以及生化和蛋白质表达研究。与未用提取物培养的缺氧细胞相比，经提取物处理的细胞显示出较少的细胞死亡、半胱天冬酶3和活性氧水平降至最低，并且G0/G1细胞周期阻滞得到缓解。此外，经提取物处理的细胞显示出Nrf2、血红素加氧酶1和金属硫蛋白的表达增加，以及缺氧诱导因子1α水平稳定。此外，在经提取物处理的细胞中，核因子-κB和肿瘤坏死因子α的水平较低。总体而言，灵芝提取物减少了缺氧诱导的细胞死亡，并增强了转录因子（HIF-1α和Nrf2），赋予了对缺氧的耐受性。
