Division of Signaling and Functional Genomics, German Cancer Research Center (DKFZ), Heidelberg, Germany.
Department of Cell and Molecular Biology, Medical Faculty Mannheim, Heidelberg University, Heidelberg, Germany.
PLoS One. 2018 Jun 28;13(6):e0199679. doi: 10.1371/journal.pone.0199679. eCollection 2018.
Lysyl oxidase-like 2 (LOXL2) is a copper-dependent monoamine oxidase that contributes to the remodelling of the extracellular matrix (ECM) by cross linkage of collagen and elastin fibres and has emerged as a potential therapeutic target in cancer and fibrosis. In the skin, LOXL2 is essential for epidermal cell polarity and differentiation. However, its role in the dermis has not been evaluated. We found that Loxl2 is dispensable for mouse dermal development, maturation and homeostasis, yet affects dermal stiffness. Neither loss of Loxl2 nor increased Loxl2 expression affected dermal architecture following treatment with the phorbol ester TPA. Furthermore, Loxl2 expression did not alter the stroma of DMBA-TPA-induced tumours. We conclude that, although Loxl2 is expressed in both dermis and epidermis, its function appears largely confined to the epidermis.
赖氨酰氧化酶样蛋白 2（LOXL2）是一种铜依赖性单胺氧化酶，通过交联胶原和弹性纤维参与细胞外基质（ECM）的重塑，并已成为癌症和纤维化的潜在治疗靶点。在皮肤中，LOXL2 对于表皮细胞极性和分化至关重要。然而，其在真皮中的作用尚未得到评估。我们发现，Loxl2 对于小鼠真皮的发育、成熟和内稳态并非必需，但会影响真皮的硬度。在使用佛波酯 TPA 处理后，缺失 Loxl2 或增加 Loxl2 表达均不会影响真皮结构。此外，Loxl2 表达不会改变 DMBA-TPA 诱导的肿瘤的基质。我们得出结论，尽管 Loxl2 在真皮和表皮中均有表达，但它的功能似乎主要局限于表皮。
