Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.
Department of Molecular Biotechnology and Bioinformatics, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand; Center for Genomics and Bioinformatics Research, Faculty of Science, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand.
Gene. 2018 Oct 20;674:170-177. doi: 10.1016/j.gene.2018.06.081. Epub 2018 Jun 25.
Ribosome: machinery in control of messenger RNA's (mRNAs) and several ribosomal proteins are in the small and large subunit of the ribosome. Various aspects of ribosomal proteins have related to cell growth, cell cycle, and diseases. Ribosomal protein L10A (RpL10A) in shrimp and fruit fly has been demonstrated to play a role in oogenesis. Interestingly, deletion RpL10A gene (RpL10Ab) germ line clone of the fruit fly showed a loss of follicle cells surrounding the egg chamber, but nurse cells appeared normal. This phenotype is reminiscent of insulin receptor mutants (InR). In contrast, over-expression of RpL10A in the eyes of the fruit flies resulted in abnormal ommatidia with a loss of red pigment in the center of the eyes. In this study, the abnormal rearrangements of nuclei and lack of cell membranes in those eyes were demonstrated. Furthermore, the expression of InR gene and the InR protein were extensively increased as determined by real-time PCR and immunohistochemistry, respectively. In addition, some insulin signaling mediators were also detected. The Akt and FOXO proteins were highly phosphorylated in the RpL10A over-expressed mutant. The results revealed that RpL10A induced over-expression of the insulin receptor and consequently activated in insulin signaling pathway which affects cell proliferation and we suggest RpL10A stimulates cell proliferation via the insulin signaling pathway.
控制信使 RNA（mRNA）的机器和几个核糖体蛋白存在于核糖体的小亚基和大亚基中。核糖体蛋白的各个方面都与细胞生长、细胞周期和疾病有关。虾和果蝇中的核糖体蛋白 L10A（RpL10A）已被证明在卵子发生中发挥作用。有趣的是，果蝇的 RpL10A 基因（RpL10Ab）生殖系克隆缺失导致卵室周围的滤泡细胞丢失，但营养细胞看起来正常。这种表型让人联想到胰岛素受体突变体（InR）。相比之下，果蝇眼睛中 RpL10A 的过度表达导致异常的小眼，眼睛中心的红色素丢失。在这项研究中，证明了这些眼睛中的核异常重排和缺乏细胞膜。此外，通过实时 PCR 和免疫组织化学分别确定了 InR 基因和 InR 蛋白的广泛表达增加。此外，还检测到一些胰岛素信号转导介质。在 RpL10A 过表达突变体中，Akt 和 FOXO 蛋白高度磷酸化。结果表明，RpL10A 诱导胰岛素受体的过度表达，进而激活胰岛素信号通路，影响细胞增殖，我们推测 RpL10A 通过胰岛素信号通路刺激细胞增殖。
