Pal Ajay, Kumar Suneel, Jain Suman, Nag Tapas C, Mathur Rashmi
J Nanosci Nanotechnol. 2018 Oct 1;18(10):6756-6764. doi: 10.1166/jnn.2018.15820.
The present study aimed to evaluate the effect of iron oxide nanoparticles (IONPs) along with electromagnetic fields (MF) exposure on spontaneous and induced axonal sprouting after spinal cord injury (SCI). Adult male Wistar rats were subjected to spinal cord transection at the T13 segment. The IONP (25 μg/mL) embedded in 3% agarose gel was implanted at the injury site and subsequently exposed to MF (50 Hz, 17.96 μT, 2 hours/day for 5 weeks). Histological analysis of spinal cord tissue showed a significant increase in the expression of the growth-associated protein GAP-43 and it was found to be co-localized with neuronal nuclei marker and neurofilaments. The results show sprouting from mature neurons and axons, significantly less demyelination and more myelinated fibers were evident at the lesion site. However, no motor or somatosensory evoked potential response was observed, suggesting lack of long-distance functional connectivity. These findings highlight the therapeutic potential of IONPs along with MF exposure in promoting neuroregeneration after SCI.
本研究旨在评估氧化铁纳米颗粒（IONPs）以及暴露于电磁场（MF）对脊髓损伤（SCI）后自发和诱导性轴突发芽的影响。成年雄性Wistar大鼠在T13节段进行脊髓横断。将嵌入3%琼脂糖凝胶中的IONP（25μg/mL）植入损伤部位，随后暴露于MF（50Hz，17.96μT，每天2小时，共5周）。脊髓组织的组织学分析显示，生长相关蛋白GAP-43的表达显著增加，并且发现它与神经元核标记物和神经丝共定位。结果显示成熟神经元和轴突发芽，损伤部位明显脱髓鞘减少，有更多有髓纤维。然而，未观察到运动或体感诱发电位反应，提示缺乏长距离功能连接。这些发现突出了IONPs与MF暴露在促进SCI后神经再生方面的治疗潜力。
