Li Xigong, Fu Jing, Guan Ming, Shi Haifei, Pan Wenming, Lou Xianfeng
Department of Orthopedics, The First Affiliated Hospital of Zhejiang University, Hangzhou, Zhejiang Province, China.
Department of Stomatology, Xixi Hospital, Hangzhou, Zhejiang Province, China.
Neural Regen Res. 2024 Sep 1;19(9):2050-2056. doi: 10.4103/1673-5374.390953. Epub 2023 Dec 15.
JOURNAL/nrgr/04.03/01300535-202409000-00038/figure1/v/2024-01-16T170235Z/r/image-tiff Previous studies have shown that Biochanin A, a flavonoid compound with estrogenic effects, can serve as a neuroprotective agent in the context of cerebral ischemia/reperfusion injury; however, its effect on spinal cord injury is still unclear. In this study, a rat model of spinal cord injury was established using the heavy object impact method, and the rats were then treated with Biochanin A (40 mg/kg) via intraperitoneal injection for 14 consecutive days. The results showed that Biochanin A effectively alleviated spinal cord neuronal injury and spinal cord tissue injury, reduced inflammation and oxidative stress in spinal cord neurons, and reduced apoptosis and pyroptosis. In addition, Biochanin A inhibited the expression of inflammasome-related proteins (ASC, NLRP3, and GSDMD) and the Toll-like receptor 4/nuclear factor-κB pathway, activated the Nrf2/heme oxygenase 1 signaling pathway, and increased the expression of the autophagy markers LC3 II, Beclin-1, and P62. Moreover, the therapeutic effects of Biochanin A on early post-spinal cord injury were similar to those of methylprednisolone. These findings suggest that Biochanin A protected neurons in the injured spinal cord through the Toll-like receptor 4/nuclear factor κB and Nrf2/heme oxygenase 1 signaling pathways. These findings suggest that Biochanin A can alleviate post-spinal cord injury at an early stage.
期刊/nrgr/04.03/01300535 - 202409000 - 00038/figure1/v/2024 - 01 - 16T170235Z/r/image - tiff 先前的研究表明，具有雌激素样作用的黄酮类化合物染料木黄酮A在脑缺血/再灌注损伤的情况下可作为神经保护剂；然而，其对脊髓损伤的影响仍不清楚。在本研究中，采用重物撞击法建立大鼠脊髓损伤模型，然后通过腹腔注射染料木黄酮A（40mg/kg）连续治疗大鼠14天。结果表明，染料木黄酮A有效减轻脊髓神经元损伤和脊髓组织损伤，降低脊髓神经元的炎症和氧化应激，减少细胞凋亡和焦亡。此外，染料木黄酮A抑制炎性小体相关蛋白（ASC、NLRP3和GSDMD）的表达以及Toll样受体4/核因子κB通路，激活Nrf2/血红素加氧酶1信号通路，并增加自噬标志物LC3 II、Beclin - 1和P62的表达。此外，染料木黄酮A对脊髓损伤早期的治疗效果与甲基强的松龙相似。这些发现表明，染料木黄酮A通过Toll样受体4/核因子κB和Nrf2/血红素加氧酶1信号通路保护受损脊髓中的神经元。这些发现表明，染料木黄酮A可在早期减轻脊髓损伤后反应。
