Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI 53706, United States.
Department of Ophthalmology and Visual Sciences, University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI 53706, United States; McPherson Eye Research Institute, University of Wisconsin-Madison, Madison, WI 53706, United States.
J Neuroimmunol. 2018 Sep 15;322:26-35. doi: 10.1016/j.jneuroim.2018.06.007. Epub 2018 Jun 11.
The purpose of this study was to characterize cell-specific expression patterns of Toll-like receptors (TLR) in non-human primate (NHP) neural retina tissue. TLR 4, 5, 6, and 7 proteins were detected by immunblotting of macaque retina tissue lysates and quantitative PCR (qPCR) demonstrated TLRs 4-7 mRNA expression. Immunofluorescence (IF) microscopy detected TLRs 4-7 in multiple cell types in macaque neural retina including Muller, retinal ganglion cells (RGC), amacrine, and bipolar cells. These results demonstrate that TLRs 4-7 are constitutively expressed by neurons in the NHP retina raising the possibility that these cells could be involved in retinal innate inflammatory responses.
本研究旨在描述 Toll 样受体（TLR）在非人灵长类（NHP）神经视网膜组织中的细胞特异性表达模式。通过免疫印迹检测食蟹猴视网膜组织裂解物中的 TLR4、5、6 和 7 蛋白，并通过定量 PCR（qPCR）证明 TLRs 4-7 mRNA 的表达。免疫荧光（IF）显微镜检测到 TLRs 4-7 在食蟹猴神经视网膜中的多种细胞类型中表达，包括 Muller 细胞、视网膜神经节细胞（RGC）、无长突细胞和双极细胞。这些结果表明 TLRs 4-7 在 NHP 视网膜的神经元中持续表达，这增加了这些细胞可能参与视网膜固有炎症反应的可能性。
