冠状病毒传染性支气管炎病毒基因组RNA测序：mRNA D的5'“独特”区域中的三个开放阅读框。

Sequencing of coronavirus IBV genomic RNA: three open reading frames in the 5' 'unique' region of mRNA D.

作者信息

Boursnell M E, Binns M M, Brown T D

出版信息

J Gen Virol. 1985 Oct;66 ( Pt 10):2253-8. doi: 10.1099/0022-1317-66-10-2253.

Abstract

The nucleotide sequence of a genomic cDNA clone corresponding to the 5' terminal domain of mRNA D of the Beaudette strain of infectious bronchitis virus (IBV) has been determined. This region contains three open reading frames which predict polypeptides of molecular weights 6700 (6.7K), 7.4K and 12.4K. The predicted 12.4K polypeptide has a codon usage very similar to that predicted for the products of the IBV nucleocapsid, membrane and spike genes. The sequence also predicts a hydrophobic, potentially membrane-anchoring, region in the N terminal half of the 12.4K polypeptide, and a hydrophilic C terminus.

摘要

已确定了与传染性支气管炎病毒（IBV）博德特毒株mRNA D的5'末端结构域相对应的基因组cDNA克隆的核苷酸序列。该区域包含三个开放阅读框，预测分子量分别为6700（6.7K）、7.4K和12.4K的多肽。预测的12.4K多肽的密码子使用情况与IBV核衣壳、膜和刺突基因产物的预测情况非常相似。该序列还预测在12.4K多肽的N端一半中有一个疏水的、可能是膜锚定的区域，以及一个亲水性的C末端。