Sokolov Igor, Dokukin Maxim E
Department of Mechanical Engineering, Tufts University, Medford, MA, USA.
Department of Biomedical Engineering, Tufts University, Medford, MA, USA.
Methods Mol Biol. 2018;1814:449-468. doi: 10.1007/978-1-4939-8591-3_27.
Atomic force microscopy (AFM) indentation analysis of cells is a unique method of measuring stiffness of the cell body and physical properties of its pericellular coat. These cell parameters correlate with cells of abnormality and diseases. Viable biological cells can be studied with this method directly in a culture dish with no special preparation. Here we describe a step-by-step method to analyze the AFM force-indentation curves to derive cell mechanics (the modulus of elasticity of the cell body) and the parameters of the pericellular coat (density and the thickness of the coat layer). Technical details, potential difficulties, and points of special attention are described.
原子力显微镜（AFM）对细胞的压痕分析是一种测量细胞体刚度及其细胞外被物理性质的独特方法。这些细胞参数与异常细胞和疾病相关。使用这种方法可以直接在培养皿中对活的生物细胞进行研究，无需特殊准备。在此，我们描述一种逐步分析AFM力-压痕曲线的方法，以得出细胞力学（细胞体的弹性模量）和细胞外被的参数（被覆层的密度和厚度）。文中还描述了技术细节、潜在困难及特别需要注意的要点。
