Jin L, Liu Y, Wang X, Qi X
a Department of Pathology , Affiliated Hospital of Jiangnan University (The Fourth People's Hospital of WuXi) , PR China.
Biotech Histochem. 2018;93(5):364-372. doi: 10.1080/10520295.2018.1444790. Epub 2018 Jun 29.
We investigated the expression of napsin A, TTF1, SPA and CK7 in primary lung adenocarcinoma (ADC) and compared antibody specificity and sensitivity. We used immunohistochemistry (IHC) using antibodies directed against napsin A, TTF1, SPA, and CK7 on cancer tissue from 454 patients diagnosed with lung cancer. In primary lung ADC, napsin A exhibited a sensitivity of 87.25% and specificity of 95.02%, TTF1 exhibited a sensitivity of 80.72% and specificity of 90.48%, SPA exhibited a sensitivity of 60.46% and specificity of 94.87% and CK7 exhibited a sensitivity of 100% and specificity of 80.31%. The order of sensitivity of the four indicators was CK7 > napsin A > TTF1 > SPA, and the order of specificity was napsin A > SPA > TTF1 > CK7. We can select effective indicators for diagnostic and differential diagnostic purposes based on differences in sensitivity and specificity.
我们研究了 napsin A、TTF1、SPA 和 CK7 在原发性肺腺癌（ADC）中的表达情况，并比较了抗体的特异性和敏感性。我们对 454 例肺癌确诊患者的癌组织进行免疫组织化学（IHC）检测，使用针对 napsin A、TTF1、SPA 和 CK7 的抗体。在原发性肺 ADC 中，napsin A 的敏感性为 87.25%，特异性为 95.02%；TTF1 的敏感性为 80.72%，特异性为 90.48%；SPA 的敏感性为 60.46%，特异性为 94.87%；CK7 的敏感性为 100%，特异性为 80.31%。这四项指标的敏感性顺序为 CK7＞napsin A＞TTF1＞SPA，特异性顺序为 napsin A＞SPA＞TTF1＞CK7。我们可以根据敏感性和特异性的差异选择有效的指标用于诊断和鉴别诊断。
