Department of Gastrointestinal Surgery, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China.
Mol Med Rep. 2018 Sep;18(3):3020-3026. doi: 10.3892/mmr.2018.9230. Epub 2018 Jun 27.
Trophinin associated protein (TROAP) is a cytoplasmic protein required for spindle assembly and cell invasion; however, its biological function in cancer remains to be elucidated. In the present study, by analyzing three independent datasets from the Oncomine database, it was identified that TROAP mRNA expression was upregulated in gastric cancer (GC) tissues compared with normal counterparts. Furthermore, elevated expression of TROAP was associated with poor survival in patients with GC, as predicted using Kaplan‑Meier analysis. TROAP was knocked down to verify its functional role in gastric cancer cell lines, SGC‑7901 and MGC80‑3. MTT assay was used to analyze cell proliferation. Cell cycle progression, and migration and invasion were determined using flow cytometry and Transwell assay, respectively. In vitro experiments demonstrated that knockdown of TROAP significantly suppressed cell proliferation, G1 to S cell cycle transition, and the migration and invasion ability of GC cells. The results of the present study suggest that TROAP is overexpressed in GC and serves an oncogenic role in gastric cancer by affecting cell proliferation and invasion.
Trophinin 相关蛋白（TROAP）是一种细胞质蛋白，对于纺锤体组装和细胞侵袭是必需的；然而，其在癌症中的生物学功能仍有待阐明。在本研究中，通过分析 Oncomine 数据库中的三个独立数据集，鉴定出 TROAP mRNA 在胃癌（GC）组织中相较于正常组织呈上调表达。此外，Kaplan-Meier 分析预测，TROAP 的高表达与 GC 患者的不良生存相关。敲低 TROAP 以验证其在胃癌细胞系 SGC-7901 和 MGC80-3 中的功能作用。使用 MTT 分析检测细胞增殖。通过流式细胞术和 Transwell 分析分别检测细胞周期进程和迁移及侵袭。体外实验表明，敲低 TROAP 可显著抑制 GC 细胞的增殖、G1 期至 S 期细胞周期转变，以及迁移和侵袭能力。本研究的结果表明，TROAP 在 GC 中呈过表达，并通过影响细胞增殖和侵袭在胃癌中发挥致癌作用。
