NID2 可以作为一个潜在的预后预测生物标志物，并促进胃癌的侵袭和迁移。

NID2 can serve as a potential prognosis prediction biomarker and promotes the invasion and migration of gastric cancer.

机构信息

Department of Intensive Care Unit (ICU), The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, PR China.

Department of Operation Anesthesiology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian, Jiangsu, PR China.

出版信息

Pathol Res Pract. 2019 Oct;215(10):152553. doi: 10.1016/j.prp.2019.152553. Epub 2019 Jul 23.

DOI:10.1016/j.prp.2019.152553

PMID:31362888

Abstract

BACKGROUND

Nidogen-2 (NID2) is a ubiquitous component in the basement membrane and plays an important role in the development of malignant tumors. However, the specific function and mechanism of the NID2 gene in gastric cancer remains unclear. In this study, we aimed to investigate the role of NID2 in gastric cancer(GC).

METHODS

Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the expression of NID2 in 67 GC tissues and adjacent normal tissues. The relationship between NID2 expression and clinicopathological features was further analyzed. In addition, we evaluated the expression of NID2 in GC based on data from the GEPIA and Kaplan-Meier Plotter database and compared the database results with our own experimental results. Invasion and wound healing assays were used to detect the function of NID2 in MKN45 and SGC7901 cells. Finally, the NID2 network and its possible related genes are constructed by the bioinformatics framework.

RESULTS

The expression level of NID2 was found to be significantly over-expressed in gastric cancer cells and tissues compared with normal controls and positively associated with TNM stage, showing a poor prognosis of GC patients. In vitro experiments indicated that NID2 was able to promote the ability of invasion and migration in GC cells. Bioinformatics prediction showed NID2 might regulate the progression of GC via protein digestion and absorption, amoebiasis, PI3K-AKt-signaling pathway, focal adhesion and ECM-receptor interaction pathways.

CONCLUSION

Our study demonstrates that up-regulated NID2 plays an important role in promoting the invasion and migration of GC cells and has a potential of being a novel biomarker for diagnosis, treatment and prognosis of GC in the future.

摘要

背景

层粘连蛋白-2（NID2）是基底膜的普遍组成部分，在恶性肿瘤的发展中发挥重要作用。然而，NID2 基因在胃癌中的具体功能和机制尚不清楚。在这项研究中，我们旨在研究 NID2 在胃癌（GC）中的作用。

方法

使用定量实时聚合酶链反应（qRT-PCR）检测 67 例 GC 组织和相邻正常组织中 NID2 的表达。进一步分析 NID2 表达与临床病理特征的关系。此外，我们根据 GEPIA 和 Kaplan-Meier Plotter 数据库中的数据评估了 GC 中 NID2 的表达，并将数据库结果与我们自己的实验结果进行了比较。侵袭和伤口愈合试验用于检测 MKN45 和 SGC7901 细胞中 NID2 的功能。最后，通过生物信息学框架构建了 NID2 网络及其可能相关基因。

结果

与正常对照组相比，胃癌细胞和组织中 NID2 的表达水平明显升高，与 TNM 分期呈正相关，提示 GC 患者预后不良。体外实验表明，NID2 能够促进 GC 细胞的侵袭和迁移能力。生物信息学预测表明，NID2 可能通过蛋白消化吸收、变形虫病、PI3K-Akt 信号通路、焦点黏附和 ECM 受体相互作用途径调节 GC 的进展。