a Microbiology & Biotechnology Research Lab, Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi 46000, Pakistan.
b Department of Biotechnology, Quaid-i-Azam University, Islamabad 44000, Pakistan.
Genome. 2018 Jul;61(7):469-476. doi: 10.1139/gen-2017-0250. Epub 2018 Jun 29.
A pigment-producing species of Serratia was isolated from the rhizosphere of a heavy metal resistant Cannabis sativa plant growing in effluent-affected soil of Hattar Industrial Estate, Haripur, Pakistan. Here, we report the genome sequence of this bacterium, which has been identified as Serratia nematodiphila on the basis of whole genome comparison using the OrthoANI classification scheme. The bacterium exhibited diverse traits, including plant growth promotion, antimicrobial, bioremediation, and pollutant tolerance capabilities including metal tolerance, azo dye degradation, ibuprofen degradation, etc. Plant growth-promoting exoenzyme production as well as phosphate solubilisation properties were observed. Genes for phosphate solubilisation, siderophore production, and chitin destruction were identified in addition to other industrially important enzymes like nitrilase and lipase. Secondary metabolite producing apparatus for high value chemicals in the whole genome was also analysed. The number of antibiotic resistance genes was then profiled in silico, through a match with Antibiotic Resistant Gene and CAR database. This is the first report of a S. nematodiphila genome from a polluted environment. This could significantly contribute to the understanding of pollution tolerance, antibiotic resistance, association with nematodes, production of bio-pesticide, and their role in plant growth promotion.
一种产色素的沙雷氏菌（Serratia）从巴基斯坦哈普尔（Haripur）哈塔工业园区（Hattar Industrial Estate）受废水影响的土壤中具有重金属抗性的大麻（Cannabis sativa）植物根际中分离出来。在这里，我们根据基于全基因组比较的 OrthoANI 分类方案，将该细菌鉴定为食线虫沙雷氏菌（Serratia nematodiphila），并报告其基因组序列。该细菌表现出多种特性，包括促进植物生长、抗菌、生物修复以及耐受能力，如金属耐受、偶氮染料降解、布洛芬降解等。观察到了产植物生长促进外酶和磷酸盐溶解特性。除了其他工业上重要的酶如腈水解酶和脂肪酶外，还鉴定了磷酸盐溶解、铁载体生产和几丁质破坏的基因。还对整个基因组中用于生产高价值化学品的次生代谢产物产生装置进行了分析。然后通过与抗生素耐药基因和 CAR 数据库进行匹配，对抗生素耐药基因进行了计算机分析。这是从污染环境中分离出的食线虫沙雷氏菌（S. nematodiphila）基因组的首次报道。这将极大地有助于理解污染耐受、抗生素耐药性、与线虫的关联、生物农药的生产及其在植物生长促进中的作用。
