Jamil-ur-Rahman Center for Genome Research, Dr. Panjwani Centre for Molecular Medicine and Drug Research, International Center for Chemical and Biological Sciences, University of Karachi, 75270 Karachi, Pakistan.
Department of Biochemistry and Molecular Biology, Michael Smith Labs, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
Curr Protein Pept Sci. 2022;23(10):697-705. doi: 10.2174/1389203723666220727142630.
Micropollutants comprise organic/mineral substances that cause an undesirable impact on the environment, by affecting life at all scales. In this study, we explored the changes they impart on the global proteome of a soil bacterium Serratia nematodiphila MB307, for two classes of pollutants, i.e., Azo dyes (Methyl orange, Congo red) and a pharmaceutical (Ibuprofen).
The 100 μg pollutant supplemented alteration of pure S. nematodiphila MB307 culture after 24 hours of incubation at 37 °C and its control was analyzed using a differential proteomics approach. MaxQuant software with the Perseus package was used for data analysis purposes.
Prominently, ribosomal proteins and chaperones were up or downregulated in the whole cell and membranous fraction.
This illustrates dynamic protein production adaptation of bacteria, to cope with stress and cell growth/division trade-off for survival. A collective pattern of survival under stress or pollution resistance could not be decrypted for all classes of pollutants, portraying dissimilar mechanisms of coping with differently structured pollutant moieties.
微污染物包括有机/无机物质，通过影响所有尺度的生命，对环境造成不良影响。在这项研究中，我们研究了两类污染物，即偶氮染料（甲基橙、刚果红）和一种药物（布洛芬），它们对土壤细菌食线虫沙雷氏菌 MB307 全球蛋白质组的影响。
在 37°C 下孵育 24 小时后，用 100μg 污染物对纯食线虫沙雷氏菌 MB307 培养物进行补充修饰，并对其对照进行分析，采用差异蛋白质组学方法。使用 MaxQuant 软件和 Perseus 软件包进行数据分析。
明显的是，核糖体蛋白和伴侣蛋白在全细胞和膜部分被上调或下调。
这说明了细菌对压力和细胞生长/分裂权衡的动态蛋白质产生适应，以维持生存。对于所有类别的污染物，都无法解密其在压力下或抗污染的集体生存模式，表明它们对不同结构的污染物部分有不同的应对机制。
