三维干细胞类器官培养中细胞分裂的实时成像。
Live imaging of cell division in 3D stem-cell organoid cultures.
作者信息
Bolhaqueiro Ana C F, van Jaarsveld Richard H, Ponsioen Bas, Overmeer René M, Snippert Hugo J, Kops Geert J P L
机构信息
Hubrecht Institute-KNAW (Royal Netherlands Academy of Arts and Sciences), Utrecht, The Netherlands; Oncode Institute, Hubrecht Institute, Utrecht, The Netherlands.
Molecular Cancer Research, University Medical Center Utrecht, Utrecht, The Netherlands; Oncode Institute, University Medical Center Utrecht, Utrecht, The Netherlands.
出版信息
Methods Cell Biol. 2018;145:91-106. doi: 10.1016/bs.mcb.2018.03.016. Epub 2018 Apr 22.
Examining cell behavior in its correct tissue context is a major challenge in cell biology. The recent development of mammalian stem cell-based organoid cultures offers exciting opportunities to visualize dynamic cellular events in a 3D tissue-like setting. We describe here an approach for live imaging of cell division processes in intestinal organoid cultures derived from human and mouse adult stem cells. These approaches can be extended to the analysis of cellular events in diseased tissue, such as patient-derived tumor organoids.
在正确的组织背景下研究细胞行为是细胞生物学中的一项重大挑战。基于哺乳动物干细胞的类器官培养技术的最新发展为在三维组织样环境中可视化动态细胞事件提供了令人兴奋的机会。我们在此描述一种对源自人和小鼠成体干细胞的肠道类器官培养物中的细胞分裂过程进行实时成像的方法。这些方法可以扩展到对患病组织中的细胞事件进行分析，例如患者来源的肿瘤类器官。
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