ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India.
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, Nanjing Agricultural University, 210095, Nanjing, China.
Plant Physiol Biochem. 2018 Sep;130:1-13. doi: 10.1016/j.plaphy.2018.06.017. Epub 2018 Jun 18.
In earlier studies at IIRR, Hyderabad, screening of ∼2000 EMS mutants of the rice variety Nagina22 (N22) resulted in the identification of 11 loss-of-function mutants with zero grain yield in Pi-deprived soil under field condition. Among these mutants, NH101 was selected for comparative analyses with N22 for various morphophysiological and/or molecular traits during growth in a hydroponic system (7 d) and in a pot soil (50% flowering) under different Pi regime. The total length of the seminal and adventitious roots, agronomic traits (panicle length and unfilled spikelet/panicle), activities of the antioxidant enzymes (SOD, POD, and APX), and the relative expression levels of the genes involved in the maintenance of Pi homeostasis (MPH) i.e., OsPHR2, SPX1/2 OsPT4, 6, and 8 showed significant increase in the Pi-deprived mutant compared with N22. Whereas, some of the traits showed significant reduction in NH101 than N22 such as number of tillers and filled spikelets/panicle, yield, contents of Pi and externally secreted APase, activity of CAT, and the relative expression levels of MPH genes i.e., OsmiR399a, OsPHO1;2, OsIPS1, OsPAP10a, OsPT2, 9, and 10. The study highlighted wide spectrum differential effects of the mutation in NH101 on various traits that play important roles governing the maintenance of Pi homeostasis. This mutant thus provides a rich repository of genetic material amenable for the identification of the genes that are pivotal for Pi use efficiency.
在 IIRR，海得拉巴的早期研究中，对水稻品种 Nagina22（N22）的约 2000 个 EMS 突变体进行了筛选，结果在田间缺磷条件下鉴定出 11 个丧失功能的突变体，其籽粒产量为零。在这些突变体中，NH101 被选择与 N22 进行比较分析，以研究在水培系统（7d）和不同磷处理下的盆栽土壤（50%开花）中生长时的各种形态生理和/或分子特性。与 N22 相比，NH101 的总穗根和不定根长度、农艺性状（穗长和未填充小穗/穗）、抗氧化酶（SOD、POD 和 APX）活性以及参与维持磷稳态的基因的相对表达水平（MPH），即 OsPHR2、SPX1/2 OsPT4、6 和 8 显著增加。然而，与 N22 相比，NH101 的一些性状如分蘖数和填充小穗/穗、产量、磷含量和外部分泌的 APase、CAT 活性以及 MPH 基因的相对表达水平（OsmiR399a、OsPHO1;2、OsIPS1、OsPAP10a、OsPT2、9 和 10）显著降低。该研究强调了 NH101 突变对各种性状的广泛差异影响，这些性状在维持磷稳态中起着重要作用。该突变体因此提供了丰富的遗传物质库，可用于鉴定对磷利用效率至关重要的基因。
