Snyder H W, Singhal M C, Yoshida L H, Jones F R
Mol Immunol. 1985 Aug;22(8):863-70. doi: 10.1016/0161-5890(85)90071-9.
A method is described for determining levels of circulating immune complexes (CIC) composed of feline leukemia virus (FeLV) antigens and corresponding antibodies in plasma of persistently-infected pet cats. The procedure is based on the ability of high-titered heterologous anti-FeLV serum to chase cat anti-FeLV IgG from dissociated CIC by successfully competing for binding of free antigen. The eluted cat antibody is then collected and quantitated. In a study of cats in the process of clearing persistent FeLV infections, measured levels of FeLV-specific CIC correlated well with fluctuating levels of free FeLV antigen and antibody. The Raji cell assay for CIC in those cats was of comparatively little value in following the clearance of the virus, presumably because that assay does not distinguish between CIC containing viral and those containing non-viral antigens. The method described can be adapted to studies of specific immune complexes associated with a variety of syndromes, provided that the antigen eliciting the immune response is known.
本文描述了一种用于测定持续感染的宠物猫血浆中由猫白血病病毒（FeLV）抗原和相应抗体组成的循环免疫复合物（CIC）水平的方法。该程序基于高滴度异源抗FeLV血清通过成功竞争游离抗原的结合，从解离的CIC中追赶猫抗FeLV IgG的能力。然后收集并定量洗脱的猫抗体。在一项对正在清除持续性FeLV感染过程中的猫的研究中，测得的FeLV特异性CIC水平与游离FeLV抗原和抗体的波动水平密切相关。在跟踪这些猫的病毒清除情况时，Raji细胞检测CIC的方法价值相对较小，可能是因为该检测方法无法区分含有病毒抗原的CIC和含有非病毒抗原的CIC。只要引发免疫反应的抗原已知，本文所述方法可适用于与各种综合征相关的特异性免疫复合物的研究。
