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克尔曼四个地区(精油)对病原菌的抗菌活性及通过气相色谱-质谱分析法测定精油的化学成分

Antibacterial Activity of (Essential Oil) Against Pathogenic Bacteria and Determination of Chemical Composition of Essential Oils by Gas Chromatography-Mass Spectrometry Analysis in Four Regions of Kerman.

作者信息

Khoddami Mojtaba, Sheikh Hosseini Mehrnaz, Hassanshahian Mehdi

机构信息

Neuroscience Research Center, Kerman University of Medical Science , Kerman , Iran.

Department of Biotechnology, Faculty Of Biological Science, Alzahra University , Tehran , Iran.

出版信息

J Diet Suppl. 2019;16(5):530-540. doi: 10.1080/19390211.2018.1472167. Epub 2018 Jun 29.

Abstract

The use of medicinal plants has been considered due to increasing bacterial resistance to antibiotics. is a plant with high potential in medicine. The aim of this study was to evaluate the antibacterial activities and gas chromatography-mass spectrometry (GC-MS) analyses of the essential oil of from four regions in Kerman: Hezar Mountain, Laleh Zar, Bidkhan, and Rabor. The essential oil of this plant was extracted by Clevenger. The antibacterial activities were evaluated against three Gram-negative bacteria () and three Gram-positive bactria (). By disk diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined. The composition of essential oil was identified by GC-MS analysis. In our study, most yield of essential oil of was 1.2% from the Laleh Zar region and the main components were Z-β-ocimene (25.4%) in Laleh Zar, linalool (17.7%) in Hezar, Z-β-ocimene (18.5%) in Bidkhan, and β-pinene (10.5%) in the Rabor region. Maximum inhibition zone by the essential oil from region Bidkhan was observed against (30 mm). The MIC value for collected from Laleh Zar was 1.25 mg/ml against and The results of this study confirm that the significant antibacterial effects of and make it a valuable compound in essential oils for pharmaceutical use and a good replacement for chemical antibiotics. Environmental conditions can result in a difference in yields and components; this can be considered significant potential for this plant.

摘要

由于细菌对抗生素的耐药性不断增加,人们开始考虑使用药用植物。[植物名称]是一种具有很高药用潜力的植物。本研究的目的是评估来自克尔曼四个地区(赫扎尔山、拉莱扎尔、比德汗和拉博尔)的[植物名称]精油的抗菌活性和气相色谱 - 质谱(GC - MS)分析。该植物的精油通过克莱文杰法提取。针对三种革兰氏阴性菌([细菌名称])和三种革兰氏阴性菌的具体名称未给出])和三种革兰氏阳性菌([细菌名称])评估了抗菌活性。通过纸片扩散法测定了最低抑菌浓度(MIC)和最低杀菌浓度(MBC)值。通过GC - MS分析确定了精油的成分。在我们的研究中,拉莱扎尔地区的[植物名称]精油产量最高,为1.2%,其主要成分在拉莱扎尔地区是Z-β-罗勒烯(25.4%),在赫扎尔地区是芳樟醇(17.7%),在比德汗地区是Z-β-罗勒烯(18.5%),在拉博尔地区是β-蒎烯(10.5%)。比德汗地区的精油对[细菌名称]观察到最大抑菌圈(30毫米)。从拉莱扎尔收集的[植物名称]对[细菌名称]和[细菌名称]的MIC值为1.25毫克/毫升。本研究结果证实了[植物名称]具有显著的抗菌作用,使其成为药用精油中的一种有价值的化合物,并且是化学抗生素的良好替代品。环境条件可能导致产量和成分的差异;这可以被认为是这种植物的巨大潜力。

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