Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology, Guilin University of Technology, Guilin, Guangxi 541004, PR China.
College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, PR China.
Sci Total Environ. 2018 Dec 1;643:842-849. doi: 10.1016/j.scitotenv.2018.06.245. Epub 2018 Jun 27.
The dynamic response of biofilm microbial ecology to para-chloronitrobenzene (p-CNB) biodegradation was systematically evaluated according to the composition and loading of electron acceptors and H availability (controlled by H pressure) in a hydrogen-based, denitrifying and sulfate-reducing membrane biofilm reactor (MBfR). To accomplish this, a laboratory-scale MBfR was set up and operated with different influent p-CNB concentrations (0, 2, and 5 mg p-CNB/L) and H pressures (0.04 and 0.05 MPa). Polymerase chain reaction-denaturing gel electrophoresis (PCR-DGGE) and cloning were then applied to investigate the bacterial diversity response of biofilm during p-CNB biodegradation. The results showed that denitrification and sulfate reduction largely controlled the total demand for H. Additionally, the DGGE fingerprint demonstrated that the addition of p-CNB, which acted as an electron acceptor, was a critical factor in the dynamics of the MBfR biofilm microbial ecology. The presence of p-CNB also had a more advantageous effect on the biofilm microbial community. Additionally, clone library analysis showed that Proteobacteria (especially beta- and gamma-) comprised the majority of the microbial biofilm response to p-CNB biodegradation, and that Pseudomonas sp. (Gammaproteobacteria) played a significant role in the biotransformation of p-CNB to aniline.
根据氢自养反硝化-硫酸盐还原膜生物膜反应器（MBfR）中电子受体的组成和负载以及 H 供应（由 H 压力控制），系统评估了生物膜微生物生态学对邻氯硝基苯（p-CNB）生物降解的动态响应。为此，建立了一个实验室规模的 MBfR，并在不同的进水 p-CNB 浓度（0、2 和 5mg p-CNB/L）和 H 压力（0.04 和 0.05MPa）下运行。然后应用聚合酶链反应-变性凝胶电泳（PCR-DGGE）和克隆技术来研究 p-CNB 生物降解过程中生物膜的细菌多样性响应。结果表明，反硝化和硫酸盐还原在很大程度上控制了 H 的总需求。此外，DGGE 指纹图谱表明，作为电子受体的 p-CNB 的添加是 MBfR 生物膜微生物生态学动态的关键因素。p-CNB 的存在对生物膜微生物群落也有更有利的影响。此外，克隆文库分析表明，变形菌门（尤其是β-和γ-变形菌）构成了生物膜对 p-CNB 生物降解的主要微生物响应，假单胞菌属（γ-变形菌）在 p-CNB 向苯胺的生物转化中发挥了重要作用。
