大鼠原代神经胶质培养细胞中酚磺基转移酶的存在情况。

Occurrence of phenolsulfotransferase in primary glial culture cells of rat.

作者信息

Yu P H, Walz W

出版信息

Neurochem Res. 1985 Jul;10(7):983-92. doi: 10.1007/BF00964634.

Abstract

Phenolsulfotransferase (PST) activity towards phenol and monoamines was determined in rat brain and in primary cultures of rat astrocytes. The pH requirement, Km values and the proportion of PST activity with respect to phenol and dopamine as substrates were similar between PST from the glial cells and the rat cortex. The enzyme activity increased with age in the brain of older animals, and also with increasing incubation time in the primary culture of astroglia. The specific PST activity of the astroglia appeared to be higher than that of the brain enzyme. In glial cultures treated with 0.25 mM dibutyryl cyclic AMP in the same culture conditions, PST activity is suppressed to about 25% of its untreated counterpart, even though dibutyryl cyclic AMP at concentrations of ImM only slightly inhibited PST activity in vitro.

摘要

在大鼠脑和大鼠星形胶质细胞原代培养物中测定了酚磺基转移酶（PST）对苯酚和单胺的活性。神经胶质细胞和大鼠皮质中的PST在pH要求、Km值以及以苯酚和多巴胺为底物时的PST活性比例方面相似。该酶活性在老年动物脑中随年龄增加而增加，在星形胶质细胞原代培养物中也随孵育时间延长而增加。星形胶质细胞的特异性PST活性似乎高于脑酶。在相同培养条件下用0.25 mM二丁酰环磷腺苷处理的神经胶质细胞培养物中，PST活性被抑制至未处理对照的约25%，尽管1 mM浓度的二丁酰环磷腺苷在体外仅轻微抑制PST活性。