以多巴胺为底物时丙磺舒对大鼠脑酚磺基转移酶活性的影响。

Effect of probenecid on rat brain phenolsulfotransferase activity using dopamine as substrate.

作者信息

Kao G Y, Elchisak M A

出版信息

Pharmacology. 1987;34(5):259-63. doi: 10.1159/000138277.

Abstract

Dopamine sulfate (DAS) is formed by the action of phenolsulfotransferase (PST) on dopamine (DA). Since probenecid is often used to investigate the transport of monoamine acidic metabolites such as DAS from the brain, we investigated the effect of probenecid on rat brain PST activity utilizing DA as substrate. In the presence of 30 or 90 microM DA, probenecid had either no effect or a dose-related inhibitory effect on PST activity. PST activity increased at low probenecid concentrations, but decreased at high probenecid concentrations when 360 microM DA was utilized. Several conditions were found where PST activity was not affected by probenecid.

摘要

硫酸多巴胺（DAS）由酚磺基转移酶（PST）作用于多巴胺（DA）形成。由于丙磺舒常用于研究诸如DAS等单胺酸性代谢产物从脑内的转运，我们以DA为底物研究了丙磺舒对大鼠脑PST活性的影响。在存在30或90微摩尔/升DA的情况下，丙磺舒对PST活性要么没有影响，要么具有剂量相关的抑制作用。当使用360微摩尔/升DA时，在低丙磺舒浓度下PST活性增加，但在高丙磺舒浓度下PST活性降低。发现了几种丙磺舒不影响PST活性的情况。