长期培养的神经胶质细胞中的A型和B型单胺氧化酶

Type A and B monoamine oxidase in glial cells in long-term culture.

作者信息

Yu P H, Hertz L

出版信息

Prog Neuropsychopharmacol Biol Psychiatry. 1983;7(4-6):687-90. doi: 10.1016/0278-5846(83)90046-5.

Abstract

Monoamine oxidase (MAO) in astroglial primary cultures increased during cell development. The enzyme activity towards type B substrate was further enhanced in cultures treated with 0.25 mM dibutyl cyclic AMP (dBcAMP), although dBcAMP (1 mM) does not effect MAO activity in vitro. It has been estimated that the dBcAMP treated cultures contain 70% type A and 30% type B MAO, while in the untreated one, there was virtually only type A MAO. Following the application of Percoll density gradient centrifugation, MAO associated with different subcellular fractions was obtained. The dBcAMP treated fractions were slightly heavier than the untreated ones.

摘要

星形胶质细胞原代培养物中的单胺氧化酶（MAO）在细胞发育过程中增加。在用0.25 mM二丁酰环磷酸腺苷（dBcAMP）处理的培养物中，对B型底物的酶活性进一步增强，尽管dBcAMP（1 mM）在体外不影响MAO活性。据估计，用dBcAMP处理的培养物中含有70%的A型和30%的B型MAO，而在未处理的培养物中，实际上只有A型MAO。应用Percoll密度梯度离心后，获得了与不同亚细胞组分相关的MAO。用dBcAMP处理的组分比未处理的组分略重。