经皮电穿孔主动给予全长 DNA Aβ42 免疫可在野生型小鼠中诱导高抗 Aβ 抗体水平。
Intradermal active full-length DNA Aβ42 immunization via electroporation leads to high anti-Aβ antibody levels in wild-type mice.
机构信息
Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center Dallas, USA.
Department of Neurology and Neurotherapeutics, University of Texas Southwestern Medical Center Dallas, USA.
出版信息
J Neuroimmunol. 2018 Sep 15;322:15-25. doi: 10.1016/j.jneuroim.2018.05.017. Epub 2018 Jun 11.
Abstract
Aβ immunotherapies with anti-Aβ antibody responses have high potential as possible prevention treatment for Alzheimer's disease. We have previously shown that active DNA Aβ1-42 immunization via gene gun delivery led to a non-inflammatory immune response resulting in decreased Aβ levels in brains of an immunized AD mouse model. To make DNA vaccination more applicable for clinical use, we used here intradermal electroporation. With fine tuning of the electropulse parameters, high antibody levels and low levels of inflammatory cytokines in the cellular immunoassays were observed. Full-length DNA Aβ1-42 immunization delivered via electroporation has potential to be used in the clinical setting.
摘要
具有抗 Aβ 抗体反应的 Aβ 免疫疗法具有作为阿尔茨海默病预防治疗的巨大潜力。我们之前已经表明，通过基因枪传递进行主动 DNA Aβ1-42 免疫接种会导致非炎症性免疫反应，从而降低免疫接种的 AD 小鼠模型大脑中的 Aβ 水平。为了使 DNA 疫苗接种更适用于临床应用，我们在这里使用了皮内电穿孔。通过精细调整电脉冲参数，观察到细胞免疫测定中的高抗体水平和低水平的炎症细胞因子。通过电穿孔传递全长 DNA Aβ1-42 免疫接种具有在临床环境中使用的潜力。
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