DSF Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, 35121 Padova, Italy.
DAFNAE Department of Agronomy Animal Foods Natural Resources and Environment, Viale dell'Università, 16, 35020 Legnaro, University of Padova.
Fitoterapia. 2018 Sep;129:154-161. doi: 10.1016/j.fitote.2018.06.022. Epub 2018 Jun 27.
An UPLC-HR-MS metabolomics approach was used to study the effects of a 49-days oral supplementation with Polygonum cuspidatum extract in healthy rats. Multivariate analysis allowed to observe significant differences in the excretion of several markers between treated animals and control group. Among the others, the amounts of N-methyl-2-pyridone-5-carboxamide (2PY) and phenylacetylglycine (PAG) were reduced in the treated group compared to control. These compounds have been previously considered as markers of aging. Furthermore, the excretion of 3-hydroxysebacic acid and 4,6-dihydroxyquinoline was also changed following supplementation, although not significantly. Despite the relatively short time of treatment (7 weeks), the significant changes in the urinary levels of aging markers observed at day 49 suggests a potential role of this type of studies as a new approach in the evaluation of the anti-aging effects of plant extracts.
采用 UPLC-HR-MS 代谢组学方法研究了 49 天口服虎杖提取物对健康大鼠的影响。多变量分析观察到治疗组动物与对照组之间几种标志物排泄的显著差异。其中，与对照组相比，N-甲基-2-吡啶酮-5-甲酰胺（2PY）和苯乙酰甘氨酸（PAG）的含量在治疗组中降低。这些化合物以前被认为是衰老的标志物。此外，补充后 3-羟基癸酸和 4,6-二羟基喹啉的排泄也发生了变化，但不显著。尽管治疗时间相对较短（7 周），但在第 49 天观察到的衰老标志物尿水平的显著变化表明，这种类型的研究作为评估植物提取物抗衰老作用的新方法具有潜在作用。
