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The ribosomal RNA promoter of Acanthamoeba castellanii determined by transcription in a cell-free system.

作者信息

Kownin P, Iida C T, Brown-Shimer S, Paule M R

出版信息

Nucleic Acids Res. 1985 Sep 11;13(17):6237-48. doi: 10.1093/nar/13.17.6237.

DOI:10.1093/nar/13.17.6237
PMID:2995922
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC321949/
Abstract

The DNA sequences required for faithful initiation of ribosomal RNA transcription were determined. BAL-31 digestion was used to modify the rDNA template by introducing deletions from its 3'- and 5'-ends. The resulting mutant DNAs were tested for template activity individually or in competition with wild type utilizing an in vitro transcription system from Acanthamoeba castellanii. The results identify the sequence extending from -31 to +8 to be absolutely required for transcription. In addition; when the region between -47 and -32 is left intact, transcription is augmented.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/dcaa00f59d58/nar00311-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/372e0b2a3dfc/nar00311-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/5de78194dab1/nar00311-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/7dd0df421d72/nar00311-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/dcaa00f59d58/nar00311-0233-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/372e0b2a3dfc/nar00311-0228-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/5de78194dab1/nar00311-0229-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/7dd0df421d72/nar00311-0230-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0055/321949/dcaa00f59d58/nar00311-0233-a.jpg

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引用本文的文献

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Site-directed photo-cross-linking of rRNA transcription initiation complexes.核糖体RNA转录起始复合物的定点光交联

本文引用的文献

1
Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter.将克隆的非洲爪蟾核糖体DNA显微注射到非洲爪蟾卵母细胞中的转录，以及一种RNA聚合酶I启动子的鉴定。
Cell. 1982 Oct;30(3):835-42. doi: 10.1016/0092-8674(82)90288-4.
2
Ribosomal RNA transcription in vitro is species specific.体外核糖体RNA转录具有物种特异性。
Nature. 1982 Mar 11;296(5853):173-4. doi: 10.1038/296173a0.
3
Sequences upstream from the T-A-T-A box are required in vivo and in vitro for efficient transcription from the adenovirus serotype 2 major late promoter.
Mol Cell Biol. 1995 Sep;15(9):4956-63. doi: 10.1128/MCB.15.9.4956.
4
Three small RNAs within the 10 kb trypanosome rRNA transcription unit are analogous to domain VII of other eukaryotic 28S rRNAs.锥虫10 kb核糖体RNA转录单元内的三种小RNA类似于其他真核生物28S核糖体RNA的结构域VII。
Nucleic Acids Res. 1986 Dec 9;14(23):9471-89. doi: 10.1093/nar/14.23.9471.
5
Factors and nucleotide sequences that direct ribosomal DNA transcription and their relationship to the stable transcription complex.指导核糖体DNA转录的因子和核苷酸序列及其与稳定转录复合体的关系。
Mol Cell Biol. 1986 Oct;6(10):3451-62. doi: 10.1128/mcb.6.10.3451-3462.1986.
6
Localization of the in vivo and in vitro transcription initiation site and comparative analysis of the flanking sequences in the two main size classes of Ascaris lumbricoides rDNA.蛔虫核糖体DNA两种主要大小类别的体内和体外转录起始位点的定位及侧翼序列的比较分析。
Nucleic Acids Res. 1987 Aug 25;15(16):6515-38. doi: 10.1093/nar/15.16.6515.
7
Effects of single-base substitutions within the Acanthamoeba castellanii rRNA promoter on transcription and on binding of transcription initiation factor and RNA polymerase I.棘阿米巴原虫rRNA启动子内单碱基替换对转录以及转录起始因子与RNA聚合酶I结合的影响。
Mol Cell Biol. 1988 Feb;8(2):747-53. doi: 10.1128/mcb.8.2.747-753.1988.
8
Events during eucaryotic rRNA transcription initiation and elongation: conversion from the closed to the open promoter complex requires nucleotide substrates.真核生物核糖体RNA转录起始和延伸过程中的事件：从封闭启动子复合物转变为开放启动子复合物需要核苷酸底物。
Mol Cell Biol. 1988 May;8(5):1940-6. doi: 10.1128/mcb.8.5.1940-1946.1988.
9
Structural determinant of the species-specific transcription of the mouse rRNA gene promoter.小鼠rRNA基因启动子物种特异性转录的结构决定因素。
Mol Cell Biol. 1989 Jan;9(1):349-53. doi: 10.1128/mcb.9.1.349-353.1989.
10
The mouse ribosomal DNA promoter has more stringent requirements in vivo than in vitro.小鼠核糖体DNA启动子在体内比在体外有更严格的要求。
Mol Cell Biol. 1990 Sep;10(9):4970-3. doi: 10.1128/mcb.10.9.4970-4973.1990.
腺病毒2型主要晚期启动子在体内和体外进行有效转录时，需要T-A-T-A框上游的序列。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7132-6. doi: 10.1073/pnas.79.23.7132.
4
Human ribosomal RNA gene: nucleotide sequence of the transcription initiation region and comparison of three mammalian genes.人类核糖体RNA基因：转录起始区域的核苷酸序列及三种哺乳动物基因的比较
Proc Natl Acad Sci U S A. 1982 May;79(10):3092-6. doi: 10.1073/pnas.79.10.3092.
5
Organization and expression of eucaryotic split genes coding for proteins.编码蛋白质的真核生物断裂基因的组织与表达。
Annu Rev Biochem. 1981;50:349-83. doi: 10.1146/annurev.bi.50.070181.002025.
6
Spacer sequences regulate transcription of ribosomal gene plasmids injected into Xenopus embryos.间隔序列调控注入非洲爪蟾胚胎的核糖体基因质粒的转录。
Cell. 1983 Oct;34(3):989-96. doi: 10.1016/0092-8674(83)90556-1.
7
Transcription by RNA polymerase III.由RNA聚合酶III进行转录。
Curr Top Dev Biol. 1983;18:59-88. doi: 10.1016/s0070-2153(08)60579-7.
8
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Proc Natl Acad Sci U S A. 1984 Apr;81(7):2137-41. doi: 10.1073/pnas.81.7.2137.
9
The origin of the rRNA precursor from Xenopus borealis, analysed in vivo and in vitro.对来自北方爪蟾的rRNA前体的起源进行体内和体外分析。
Nucleic Acids Res. 1983 Dec 10;11(23):8167-81. doi: 10.1093/nar/11.23.8167.
10
Determination of the promoter region of mouse ribosomal RNA gene by an in vitro transcription system.利用体外转录系统确定小鼠核糖体RNA基因的启动子区域。
Proc Natl Acad Sci U S A. 1984 Jan;81(2):299-303. doi: 10.1073/pnas.81.2.299.