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对来自北方爪蟾的rRNA前体的起源进行体内和体外分析。

The origin of the rRNA precursor from Xenopus borealis, analysed in vivo and in vitro.

作者信息

McStay B, Bird A

出版信息

Nucleic Acids Res. 1983 Dec 10;11(23):8167-81. doi: 10.1093/nar/11.23.8167.

DOI:10.1093/nar/11.23.8167
PMID:6324076
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC326573/
Abstract

We have determined the origin of the major transcript of Xenopus borealis rDNA by the use of an SI nuclease protection assay. The DNA surrounding the origin of this transcript was sequenced, and the region upstream of the origin was shown to have strong sequence homology with that region from X.laevis rDNA. We have also demonstrated faithful transcription from this origin using cloned X.borealis rDNA in an extract derived from X. laevis culture cells. This in vitro transcription was insensitive to 100 micrograms/ml alpha-amanatin, suggesting that it was mediated by RNA polymerase 1.

摘要

我们通过使用S1核酸酶保护试验确定了非洲爪蟾rDNA主要转录本的起源。对该转录本起源周围的DNA进行了测序，结果表明该起源上游区域与非洲爪蟾rDNA的相应区域具有很强的序列同源性。我们还利用从非洲爪蟾培养细胞中提取的提取物，通过克隆的非洲爪蟾rDNA证明了该起源处的转录是忠实的。这种体外转录对100微克/毫升的α-鹅膏蕈碱不敏感，这表明它是由RNA聚合酶I介导的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9898/326573/06336faeeed7/nar00368-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9898/326573/7ba0a747a571/nar00368-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9898/326573/43536e001b08/nar00368-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9898/326573/06336faeeed7/nar00368-0066-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9898/326573/7ba0a747a571/nar00368-0063-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9898/326573/43536e001b08/nar00368-0065-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9898/326573/06336faeeed7/nar00368-0066-a.jpg

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引用本文的文献

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3
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本文引用的文献

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Transcription of mouse rRNA genes by RNA polymerase I: in vitro and in vivo initiation and processing sites.RNA聚合酶I对小鼠rRNA基因的转录：体外和体内的起始及加工位点
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Improvements of DNA sequencing gels.
蛔虫核糖体DNA两种主要大小类别的体内和体外转录起始位点的定位及侧翼序列的比较分析。
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DNA sequences for typical ribosomal gene spacers from Xenopus laevis and Xenopus borealis.非洲爪蟾和北美爪蟾典型核糖体基因间隔区的DNA序列。
Nucleic Acids Res. 1987 Apr 24;15(8):3623-4. doi: 10.1093/nar/15.8.3623.
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Effects of single-base substitutions within the Acanthamoeba castellanii rRNA promoter on transcription and on binding of transcription initiation factor and RNA polymerase I.棘阿米巴原虫rRNA启动子内单碱基替换对转录以及转录起始因子与RNA聚合酶I结合的影响。
Mol Cell Biol. 1988 Feb;8(2):747-53. doi: 10.1128/mcb.8.2.747-753.1988.
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Transcription of eukaryotic ribosomal RNA gene.真核生物核糖体RNA基因的转录
Mol Cell Biochem. 1986 Apr;70(1):11-20. doi: 10.1007/BF00233800.
7
The ribosomal RNA promoter of Acanthamoeba castellanii determined by transcription in a cell-free system.
Nucleic Acids Res. 1985 Sep 11;13(17):6237-48. doi: 10.1093/nar/13.17.6237.
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An RNA polymerase I promoter located in the CHO and mouse ribosomal DNA spacers: functional analysis and factor and sequence requirements.位于中国仓鼠卵巢细胞（CHO）和小鼠核糖体DNA间隔区的RNA聚合酶I启动子：功能分析以及因子和序列要求
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DNA测序凝胶的改进。
Anal Biochem. 1981 Aug;115(2):450-7. doi: 10.1016/0003-2697(81)90031-2.
4
Transcription of cloned Xenopus laevis ribosomal DNA microinjected into Xenopus oocytes, and the identification of an RNA polymerase I promoter.将克隆的非洲爪蟾核糖体DNA显微注射到非洲爪蟾卵母细胞中的转录，以及一种RNA聚合酶I启动子的鉴定。
Cell. 1982 Oct;30(3):835-42. doi: 10.1016/0092-8674(82)90288-4.
5
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Nature. 1982 Mar 11;296(5853):173-4. doi: 10.1038/296173a0.
6
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