Wolf S S, Moody T W
Peptides. 1985;6 Suppl 1:111-4. doi: 10.1016/0196-9781(85)90018-x.
Binding sites in the rat forebrain were characterized using (125I-Tyr4)bombesin as a receptor probe. Pharmacology experiments indicate that gastrin releasing peptide (GRP) and the GRP fragments GRP as well as Ac-GRP inhibited radiolabeled (Tyr4)bombesin binding with high affinity. Biochemistry experiments indicated that heat, N-ethyl maleimide or trypsin greatly reduced radiolabeled (Tyr4)bombesin binding. Also, autoradiographic studies indicated that highest grain densities were present in the stria terminalis, periventricular and suprachiasmatic nucleus of the hypothalamus, dorsomedial and rhomboid thalamus, dentate gyrus, hippocampus and medial amygdaloid nucleus. The data suggest that CNS protein receptors, which are discretely distributed in the rat forebrain, may mediate the action of endogenous GRP/bombesin-like peptides.
使用（125I-Tyr4）蛙皮素作为受体探针来表征大鼠前脑的结合位点。药理学实验表明，胃泌素释放肽（GRP）及其GRP片段GRP以及Ac-GRP以高亲和力抑制放射性标记的（Tyr4）蛙皮素结合。生物化学实验表明，加热、N-乙基马来酰亚胺或胰蛋白酶可大大降低放射性标记的（Tyr4）蛙皮素结合。此外，放射自显影研究表明，最高的颗粒密度出现在终纹、下丘脑室周和视交叉上核、背内侧和菱形丘脑、齿状回、海马和内侧杏仁核中。数据表明，在大鼠前脑中离散分布的中枢神经系统蛋白受体可能介导内源性GRP/蛙皮素样肽的作用。
