Faustini Massimo, Curone Giulio, Torre Maria L, Vigo Daniele
Dipartimento di Medicina Veterinaria, Università degli Studi di Milano, Milan, Italy.
Dipartimento di Scienze del Farmaco, Università degli Studi di Pavia, Pavia, Italy.
Methods Mol Biol. 2018;1817:89-93. doi: 10.1007/978-1-4939-8600-2_9.
A protocol for the encapsulation in sodium alginate of granulosa cells in primary culture and co-culture of oocyte-cumulus complexes is reported. Sodium alginate forms strong gels when jellified with barium ions, allowing the self-organization of cells into a 3D structure. This method of encapsulation is simple and cheap, allowing the culture of cells in a 3-dimensional fashion.
