Leoni Giovanni Giuseppe, Naitana Salvatore
Department of Veterinary Medicine, University of Sassari, Sassari, Italy.
Methods Mol Biol. 2018;1817:95-106. doi: 10.1007/978-1-4939-8600-2_10.
The functional cooperation between the oocyte and its surrounding granulosa cells is mandatory to oocyte growth and acquisition of developmental competence in mammalian species. Availability of in vitro methods for culture granulosa cells is important in understanding the biology of these cells and the response of maturing oocyte to in vitro culture and co-culture. Using the ovine as a model the ensuing chapter describes methods for primary culture of ovine granulosa cells and their co-culture with theca cells or oocytes that allow to mimic the molecular interactions between somatic cells and maturing oocyte and improve oocyte quality.
在哺乳动物中，卵母细胞与其周围颗粒细胞之间的功能协作对于卵母细胞生长及发育能力的获得至关重要。具备体外培养颗粒细胞的方法，对于理解这些细胞的生物学特性以及成熟卵母细胞对体外培养和共培养的反应具有重要意义。本章以绵羊为模型，描述了绵羊颗粒细胞原代培养及其与卵泡膜细胞或卵母细胞共培养的方法，这些方法能够模拟体细胞与成熟卵母细胞之间的分子相互作用并提高卵母细胞质量。
