紫外线诱导的噬菌体S13诱变可在缺乏大肠杆菌RecA和UmuC蛋白的情况下发生。
UV-induced mutagenesis of phage S13 can occur in the absence of the RecA and UmuC proteins of Escherichia coli.
作者信息
Tessman I
出版信息
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6614-8. doi: 10.1073/pnas.82.19.6614.
Abstract
The UV-induced mutagenesis of phage S13 that accompanies Weigle repair is known to require the products of the recA and umuDC genes, as does the UV-induced mutagenesis of the Escherichia coli chromosome. I found that UV-induced mutagenesis of phage S13 occurred in the absence of both the RecA and UmuC functions when the irradiated phage was photoreactivated. Furthermore, UV-induced phage mutations were produced in a recA- umuC- cell even without photoreactivation and in the absence of any other known UV repair mechanism, at a frequency 29% of that found after photoreactivation and 7% of that found after Weigle repair, implying that DNA synthesis can proceed past a dimer at an unexpectedly high frequency even when unaided by the UmuC-RecA SOS repair functions. The unaided DNA synthesis appears capable of producing mutations in the vicinity of a pyrimidine dimer; by aiding synthesis past a dimer, a repair mechanism may disclose a mutation without having any active role in producing it.
摘要
已知伴随韦格勒修复的噬菌体S13的紫外线诱导诱变需要recA和umuDC基因的产物，大肠杆菌染色体的紫外线诱导诱变也是如此。我发现，当受照射的噬菌体进行光复活时，噬菌体S13的紫外线诱导诱变在RecA和UmuC功能均缺失的情况下发生。此外，即使没有光复活且不存在任何其他已知的紫外线修复机制，recA- umuC-细胞中也会产生紫外线诱导的噬菌体突变，其频率为光复活后发现频率的29%，韦格勒修复后发现频率的7%，这意味着即使没有UmuC-RecA SOS修复功能的辅助，DNA合成也能以出乎意料的高频率越过二聚体进行。这种无辅助的DNA合成似乎能够在嘧啶二聚体附近产生突变；通过辅助越过二聚体进行合成，一种修复机制可能会揭示一个突变，而在产生该突变过程中没有任何积极作用。
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