Molina José Manuel, Hernández Yeray Isidoro, Martín Sergio, Ferrer Otilia, Rodríguez Francisco, Ruiz Antonio
Department of Animal Pathology, Faculty of Veterinary Medicine, University of Gran Canaria, Gran Canaria, Spain.
Department of Anatomy and Compared Anatomy Pathology, Faculty of Veterinary Medicine, University of Gran Canaria, Gran Canaria, Spain.
Parasite Immunol. 2018 Sep;40(9):e12569. doi: 10.1111/pim.12569. Epub 2018 Jul 25.
The experimental protocol of immunization tested here confirms its protective effect against Haemonchus contortus in goats. This protection translated into a 65.5% mean reduction in adult worm burden after a homologous challenge, and a significant decrease (73.2%) in cumulative faecal egg counts (FECs). These parasitological findings were consistent with the levels of some biopathological parameters. Thus, the reduction in adult worms and FEC observed in immunized animals were associated with increased levels of packed cell volume as well as plasma proteins. This response seems to be related to an important increase in specific antibodies (in serum and gastric mucus) and eosinophilia in response to challenge. At the local level, a cellular response was also observed in which CD4 lymphocytes and globule leucocytes played a predominant role. Finally, it should be noted that the study of immunolocalization of proteins used in the vaccination trial suggests that these antigens have an internal location (at intestinal and reproductive tissues) in the adult worm. This observation, in conjunction with the kinetics of specific antibody levels after the challenge, suggests that these antigens may be part of excretory/secretory (E/S) products.
此处测试的免疫实验方案证实了其对山羊捻转血矛线虫的保护作用。这种保护作用表现为同源攻击后成虫负荷平均降低65.5%，以及累积粪便虫卵计数（FEC）显著减少（73.2%）。这些寄生虫学结果与一些生物病理学参数水平一致。因此，在免疫动物中观察到的成虫和FEC减少与红细胞压积水平以及血浆蛋白水平升高有关。这种反应似乎与受到攻击后特异性抗体（血清和胃黏液中）的显著增加以及嗜酸性粒细胞增多有关。在局部水平，还观察到了一种细胞反应，其中CD4淋巴细胞和球形白细胞起主要作用。最后，应该指出的是，对疫苗试验中使用的蛋白质进行免疫定位研究表明，这些抗原在成虫中位于内部（在肠道和生殖组织）。这一观察结果，结合攻击后特异性抗体水平的动力学，表明这些抗原可能是排泄/分泌（E/S）产物的一部分。
