Conde-Felipe Magnolia M, Molina José Adrián, Ruiz Antonio, Ferrer Otilia, Del Rio Mª Cristina, Carmelo Emma, Hernández-Fernaud Juan R, Rodríguez Francisco, Molina José Manuel
Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Medicine, University of Las Palmas de Gran Canaria, 35413 Gran Canaria, Spain.
UIC Zoonosis y Enfermedades Emergentes ENZOEM, Universidad de Córdoba, 14071 Córdoba, Spain.
Vaccines (Basel). 2025 Jun 29;13(7):708. doi: 10.3390/vaccines13070708.
: A comparative analysis was conducted between two immunisation protocols using different amounts of protein extracts from adult worms, purified by thiol-Sepharose chromatography (625 μg/animal vs. 200 μg/animal). These protocols involved either five or two inoculations of the immunogen, respectively. : To evaluate the level of immunoprotection, animals were challenged with L3 of two weeks after the last inoculation of the immunogen and humanely sacrificed at 8 weeks post-infection. Parasitological, biopathological, and serological parameters were monitored through the experiment. Parasite burden, abomasal-specific antibody responses, and histopathological changes were determined at the end of the trial. : The immunisation protocols resulted in similar reductions in cumulative faecal egg counts (60.5-64.9%) and the total worm burden (47.5-50%) compared to non-immunized (control) animals. Overall, these parasitological data showed an early recovery of the haematocrit (PCV) after challenge in the immunised groups relative to control. Similarly, levels of -specific IgG and IgA antibodies increased in both the serum and gastric mucus of immunised groups. : These findings represent a further step towards the potential application of this type of immunogen under field conditions, as protective responses (associated with a reduction in faecal egg output) were achieved using a simplified protocol, with lower immunogen doses and fewer inoculations required to induce immunoprotection, thereby mitigating the pathological effects of the parasite and reducing its ability to spread and infect susceptible hosts.
对两种免疫方案进行了比较分析，这两种方案使用了通过硫醇-琼脂糖凝胶色谱法纯化的来自成虫的不同量的蛋白质提取物（625μg/动物与200μg/动物）。这些方案分别涉及对免疫原进行五次或两次接种。为了评估免疫保护水平，在最后一次接种免疫原两周后用L3对动物进行攻击，并在感染后8周进行人道处死。在整个实验过程中监测寄生虫学、生物病理学和血清学参数。在试验结束时测定寄生虫负荷、皱胃特异性抗体反应和组织病理学变化。与未免疫（对照）动物相比，免疫方案导致累积粪便虫卵计数（60.5 - 64.9%）和总虫负荷（47.5 - 50%）有相似程度的降低。总体而言，这些寄生虫学数据表明，与对照组相比，免疫组在攻击后血细胞比容（PCV）能较早恢复。同样，免疫组血清和胃黏液中特异性IgG和IgA抗体水平均升高。这些发现代表了朝着这种类型的免疫原在野外条件下的潜在应用又迈进了一步，因为使用简化方案实现了保护性反应（与粪便虫卵排出量减少相关），诱导免疫保护所需的免疫原剂量更低且接种次数更少，从而减轻了寄生虫的病理影响并降低了其传播和感染易感宿主的能力。
