State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica & Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China; School of Pharmacy, Xinxiang Medical University, Xinxiang, Henan, 453000, China.
State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica & Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.
J Neurosci Methods. 2018 Sep 1;307:60-69. doi: 10.1016/j.jneumeth.2018.06.026. Epub 2018 Jun 28.
Stereotaxic procedures in rats are frequently used in neurological research. The commonly used rat brain stereotaxic atlases areThe Rat Brain in Stereotaxic Coordinates, the second most cited book in science, and Stereotaxic Atlas of Rat Brain (in Chinese) which based on Wistar rats of 290 g and SD rats of 225 g, respectively. However, rats of different weight are frequently used, while the stereotaxic coordinates of their brain regions show obvious differences with the rats on which the atlases were based.
The craniometric parameters were measured as accurately as possible, then curve fitting equations obtained from the parameters were used to deduce the relative coordinates in rats of different weight for the first time.
The results revealed that if the coordinates of target brain region were defined as AP(a), ML(b), DV(c) in "standard" rats, the corresponding coordinates AP(a'),ML(b'),DV(c') for rats weighingm(g) were described as follows: For Wistar rats using The Rat Brain in Stereotaxic Coordinates: a' =0.138×lnm+0.1770.138×ln290+0.177×a b' =0.055×lnm+0.5150.055×ln290+0.515×b c' =0.054×lnm+0.7060.054×ln290+0.706×c-0.00012×m-290 and for SD rats using Stereotaxic Atlas of Rat Brain(in Chinese): a' =0.127×lnm+0.1760.127×ln225+0.176×a b'=0.049×lnm+0.5560.049×ln225+0.556×b c' =0.077×lnm+0.6040.077×ln225+0.604×c-0.00010×m-225 Unit of a, b, c, a', b', c' are centimeter, unit of m is gram. Comparison with existing method: The Rat Brain in Stereotaxic Coordinates is applicable for rats weighing about 290 g, while our equations extended the use of atlas to rats weighing 90-400 g.
In this manner, the application of the rat brain atlases could be extended and the results would be of great help in stereotaxic procedures.
立体定位程序在大鼠中经常用于神经科学研究。常用的大鼠脑立体定位图谱有《大鼠脑立体定位图谱》，这是科学领域第二高被引的书籍，以及《大鼠脑立体定位图谱》（中文版），分别基于 290 克的 Wistar 大鼠和 225 克的 SD 大鼠。然而，经常使用不同体重的大鼠，而其脑区的立体定位坐标与图谱所基于的大鼠明显不同。
尽可能准确地测量颅测参数，然后首次使用从参数中获得的曲线拟合方程推导出不同体重大鼠的相对坐标。
结果表明，如果目标脑区的坐标在“标准”大鼠中定义为 AP(a)、ML(b)、DV(c)，则相应的坐标 AP(a')、ML(b')、DV(c')对于体重为 m(g)的大鼠可以表示为：对于使用《大鼠脑立体定位图谱》的 Wistar 大鼠：a'=0.138×lnm+0.177，0.138×ln290+0.177×a b'=0.055×lnm+0.515，0.055×ln290+0.515×b c'=0.054×lnm+0.706，0.054×ln290+0.706×c-0.00012×m-290。对于使用《大鼠脑立体定位图谱》（中文版）的 SD 大鼠：a'=0.127×lnm+0.176，0.127×ln225+0.176×a b'=0.049×lnm+0.556，0.049×ln225+0.556×b c'=0.077×lnm+0.604，0.077×ln225+0.604×c-0.00010×m-225。a、b、c、a'、b'、c'的单位为厘米，m 的单位为克。与现有方法的比较：《大鼠脑立体定位图谱》适用于约 290 克的大鼠，而我们的方程则将图谱的应用扩展到了 90-400 克的大鼠。
通过这种方式，可以扩展大鼠脑图谱的应用，并在立体定位程序中提供很大的帮助。
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