Lirio Pedro Henrique Cassaro, Gonçalves Jessica Vaz, Filho Walter Nunes Pontara, Amancio Thamiris Alves, Carlini Juliana Tancredo, Dalpiaz Polyana Lima Meireles, Sartório Carmem Luíza, Rodrigues Livia Carla de Melo, Areas Fernando Zanela da Silva
Department of Physiological Sciences, Federal University of Espírito Santo, Vitória, Brazil.
Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha, ES, Brazil.
MethodsX. 2024 Jun 20;13:102806. doi: 10.1016/j.mex.2024.102806. eCollection 2024 Dec.
Traumatic brain injury (TBI) is a neurotrauma with a complex pathophysiology caused by an external mechanical force. This global public health problem is a leading cause of death and disability in young adults. In this scenario, many models were developed to try to simulate human TBI. The weight drop model allows the investigation of the pathophysiological cascades of TBI without surgical interference. In this protocol, a new closed-head weight-drop rat model consisting of a 48.5g weight projectile that free falls from 1.10m high onto the skull of the animals was built. We classify the present TBI model performed as moderately severe due to its mortality rate. Animals from TBI and Control (Sham) groups underwent weight for 7 days and temperature assessments within 1 hour after TBI and for 7 days. Results demonstrated that the TBI group showed less body weight gain in the days after the injury. Temperature oscillations within the first-hour post-injury and on the 3rd day after injury were observed. As the results of this study demonstrated similarity to human TBI vital parameters, this new adaptation of the Weight-drop model injury can be a suitable candidate for translational studies.•We developed a novel closed head focal traumatic brain injury using a projectile.•This TBI model does not require surgical intervention.•The validation of this method demonstrates that the vital parameters of the injured rats exhibit similarities with those of TBI patients.
创伤性脑损伤（TBI）是一种由外部机械力导致的、具有复杂病理生理学机制的神经创伤。这一全球公共卫生问题是年轻成年人死亡和残疾的主要原因。在这种情况下，人们开发了许多模型来试图模拟人类TBI。重物下落模型能够在不进行手术干预的情况下研究TBI的病理生理级联反应。在本实验方案中，构建了一种新的闭合性颅脑重物下落大鼠模型，该模型使用一个48.5克的重物从1.10米高处自由下落到动物颅骨上。由于其死亡率，我们将目前所实施的TBI模型归类为中度严重。TBI组和对照组（假手术组）的动物在TBI后1小时内及之后7天进行体重测量，并在TBI后1小时内及之后7天进行体温评估。结果表明，TBI组在受伤后的几天内体重增加较少。观察到受伤后第1小时内和受伤后第3天的体温波动。由于本研究结果显示与人类TBI的生命参数相似，这种新的重物下落模型损伤适应性可成为转化研究的合适候选模型。
•我们使用抛射体开发了一种新型闭合性颅脑局灶性创伤性脑损伤模型。
•这种TBI模型不需要手术干预。
•该方法的验证表明，受伤大鼠的生命参数与TBI患者的生命参数相似。
