重金属对培养的大鼠肾上腺皮质细胞中皮质类固醇生成的影响。

Effects of heavy metals on corticosteroid production in cultured rat adrenocortical cells.

作者信息

Nishiyama S, Nakamura K, Ogawa M

出版信息

Toxicol Appl Pharmacol. 1985 Oct;81(1):174-6. doi: 10.1016/0041-008x(85)90132-2.

Abstract

Application of 10(-4) M Zn, 10(-5) M Ba, 10(-6) M Se, 10(-6) M Cd, 10(-6) M Hg, and 10(-6) M Mn did not affect the adrenocorticotrophic hormone (ACTH)-induced steroid production in cultured adrenocortical cells. The application of 10(-5) M Pb significantly reduced the ACTH-induced steroid production in cultured cells. However, Pb did not reduce the steroidogenesis induced by dibutyryl cyclic AMP (Dbc-AMP), suggesting that the plasma membrane is the site where Pb interferes with steroid production. The morphological changes induced by the addition of ACTH or Dbc-AMP plus the test metals were similar to those induced by ACTH or Dbc-AMP alone.

摘要

应用10⁻⁴ M锌、10⁻⁵ M钡、10⁻⁶ M硒、10⁻⁶ M镉、10⁻⁶ M汞和10⁻⁶ M锰对培养的肾上腺皮质细胞中促肾上腺皮质激素（ACTH）诱导的类固醇生成没有影响。应用10⁻⁵ M铅显著降低了培养细胞中ACTH诱导的类固醇生成。然而，铅并没有降低二丁酰环磷腺苷（Dbc-AMP）诱导的类固醇生成，这表明质膜是铅干扰类固醇生成的部位。添加ACTH或Dbc-AMP加测试金属所诱导的形态变化与单独添加ACTH或Dbc-AMP所诱导的形态变化相似。