Kawamura M, Nakamichi N, Imagawa N, Tanaka Y, Tomita C, Matsuba M
Jpn J Pharmacol. 1984 Sep;36(1):35-41. doi: 10.1254/jjp.36.35.
In primary 2-day cultured bovine adrenocortical cells, adrenaline stimulated the steroidogenesis, while the effect of adrenaline did not appear in the freshly isolated cells. Thus the primary 2-day cultured cells were used to study the effect of adrenaline on steroidogenesis. Adrenaline showed the steroidogenesis-stimulating effect at concentrations higher than 10(-9) M, and the maximum effect was obtained between 10(-6) M and 10(-5) M in the primary 2-day cultured cells. The maximum effect of adrenaline was 50-70% of that of adrenocorticotropic hormone (ACTH). Noradrenaline, isoproterenol and phenylephrine also stimulated the steroidogenesis. However, the order of the potency was isoproterenol much greater than adrenaline = noradrenaline much much greater than phenylephrine. Propranolol and alprenolol inhibited the effect of adrenaline, but phenoxybenzamine and phentolamine did not inhibit the effect. Moreover, adrenaline stimulated the cyclic AMP production dose-dependently at concentrations higher than 10(-8) M. These results suggest that there are steroidogenesis-linked adrenergic receptors in primary 2-day cultured bovine adrenocortical cell membrane and that the steroidogenesis-stimulating effect of adrenaline occurs through the beta-adrenergic receptor.
在原代培养2天的牛肾上腺皮质细胞中，肾上腺素可刺激类固醇生成，而在新鲜分离的细胞中未出现这种效应。因此，使用原代培养2天的细胞来研究肾上腺素对类固醇生成的影响。在原代培养2天的细胞中，肾上腺素在浓度高于10^(-9) M时表现出刺激类固醇生成的作用，最大效应在10^(-6) M至10^(-5) M之间获得。肾上腺素的最大效应是促肾上腺皮质激素（ACTH）的50 - 70%。去甲肾上腺素、异丙肾上腺素和苯肾上腺素也刺激类固醇生成。然而，效力顺序为异丙肾上腺素远大于肾上腺素 = 去甲肾上腺素远大于苯肾上腺素。普萘洛尔和阿普洛尔抑制肾上腺素的作用，但酚苄明和酚妥拉明不抑制该作用。此外，肾上腺素在浓度高于10^(-8) M时剂量依赖性地刺激环磷酸腺苷（cAMP）的产生。这些结果表明，在原代培养2天的牛肾上腺皮质细胞膜中存在与类固醇生成相关的肾上腺素能受体，且肾上腺素刺激类固醇生成的作用是通过β - 肾上腺素能受体实现的。
