Cai Ping-Ping, Ning Zhuo, He Ze, Zhang Min, Shi Jian-Sheng
Institute of Hydrogeology and Environmental Geology, Chinese Academy of Geological Sciences, Shijiazhuang 050061, China.
School of Resources and Enviromental Engineering, Hefei University of Technology, Hefei 230009, China.
Huan Jing Ke Xue. 2018 Jul 8;39(7):3329-3338. doi: 10.13227/j.hjkx.201711233.
To design a reasonable and effective remediation scheme for soil in contaminated sites, it is necessary to understand the microbial communities in the soil. Samples were collected at different depths (0 cm to 400 cm) in four locations: one that was persistently contaminated and near an oil well, one that was historically contaminated in the middle of the site, one in a mud pit, and one in farmland. High-throughput sequencing of the V4 region of 16S rRNA in these samples was performed. In addition to physico-chemical properties of the soil, the -diversity, species composition, and differences in species between groups of microorganisms were analyzed, and a principal coordinate analysis and canonical correlation analysis were conducted. Results showed that oil and salt contents in soils were the dominant factors affecting microbial community structure. Hydrocarbon-degrading microorganisms were abundant in oil-contaminated soils, whereas halophilic hydrocarbon-degrading microorganisms were present in soils with high salt contents. Therefore, hydrocarbon-degrading microorganisms might be useful in remediation of oil-contaminated sites.
为设计出合理有效的污染场地土壤修复方案，有必要了解土壤中的微生物群落。在四个地点的不同深度（0厘米至400厘米）采集了样本：一个是持续受污染且靠近油井的地点，一个是场地中部曾受污染的地点，一个是泥坑，还有一个是农田。对这些样本中16S rRNA的V4区域进行了高通量测序。除了分析土壤的理化性质外，还分析了微生物的多样性、物种组成以及不同组之间的物种差异，并进行了主坐标分析和典范相关分析。结果表明，土壤中的油和盐含量是影响微生物群落结构的主要因素。在受油污染的土壤中，烃降解微生物丰富，而在高盐含量的土壤中存在嗜盐烃降解微生物。因此，烃降解微生物可能对油污染场地的修复有用。
