Ahady Mohammad Taghi, Hoghooghi-Rad Nasser, Madani Rasool, Esmaeili Rastaghi Ahmad Reza
Dept. of Microbiology, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Dept. of Parasitology, Pasteur Institute of Iran, Tehran, Iran.
Iran J Parasitol. 2018 Jan-Mar;13(1):39-48.
Toxoplasmosis is a parasitic disease caused by the intracellular protozoan parasite, , which can infect humans and warm-blooded animals. This infection can lead to still birth and abortion among some susceptible hosts especially sheep and human in pregnancy. Development of a vaccine against infection is very important-especially for use in immunocompromised patients, pregnant women, and sheep. Different antigens of can be potential candidates for immunization. The aims of this study were to identify the immunodominant and antigenic proteins of in sheep and man.
Tachyzoites' proteins were separated by two-dimensional polyacrylamide gel electrophoresis (2-DE), and subjected to western blot analysis probed with positive sera of sheep and human (Biotechnology Department of Pasteur Institute of Tehran, Iran, from April 2016 to March 2017). Finally, the immunoreactive proteins were identified by mass spectrometry (MALDI-TOF/MS and MS/MS) technique.
Five immunoreactive and antigenic proteins were recognized by positive sera of human and sheep. These identified proteins were Enolase 2, rhoptry protein 4 (ROP4), dense granular protein 14 (GRA14), rhoptry protein 15 (ROP15) and rhoptry protein 9 (ROP9).
The identified immunodominant proteins have potential to be used as diagnostic antigens and as diagnostic markers of infection in sheep and human.
弓形虫病是一种由细胞内原生动物寄生虫弓形虫引起的寄生虫病，可感染人类和温血动物。这种感染可导致一些易感宿主（尤其是孕期的绵羊和人类）出现死产和流产。开发针对弓形虫感染的疫苗非常重要，特别是用于免疫功能低下的患者、孕妇和绵羊。弓形虫的不同抗原可能是免疫接种的潜在候选物。本研究的目的是鉴定绵羊和人类中弓形虫的免疫显性和抗原性蛋白。
速殖子蛋白通过二维聚丙烯酰胺凝胶电泳（2-DE）分离，并用绵羊和人类的弓形虫阳性血清进行蛋白质印迹分析（伊朗德黑兰巴斯德研究所生物技术部，2016年4月至2017年3月）。最后，通过质谱（MALDI-TOF/MS和MS/MS）技术鉴定免疫反应性蛋白。
人类和绵羊的弓形虫阳性血清识别出五种免疫反应性和抗原性蛋白。这些鉴定出的蛋白是烯醇化酶2、棒状体蛋白4（ROP4）、致密颗粒蛋白14（GRA14）、棒状体蛋白15（ROP15）和棒状体蛋白9（ROP9）。
鉴定出的免疫显性蛋白有潜力用作诊断抗原以及绵羊和人类弓形虫感染的诊断标志物。
