Zheng Xiao-Li, Yu Hong-Gang
Department of Gastroenterology, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.
Oncol Lett. 2018 Jul;16(1):1163-1172. doi: 10.3892/ol.2018.8729. Epub 2018 May 16.
Wnt proteins have been reported to contribute to the progression of various types of cancer. Wnt6 is a member of the Wnt family and may promote tumorigenesis in gastrointestinal cancer and cervical cancer. In the present study, the expression of Wnt6 in human colon cancer cell lines was evaluated, in order to investigate the role of Wnt6 in the development of colon cancer. Additionally, the effects of Wnt6 upregulation or downregulation on proliferation, apoptosis, cell cycle and cell migration of colon cancer cells have been investigated. Furthermore, western blot analysis was employed to evaluate the expression of Wnt6, B-cell lymphoma 2-associated X protein (Bax), caspase-3 and matrix metalloproteinase (MMP)2. The results of the present study demonstrated that the expression of Wnt6 was increased in HCT116 and SW480 cells compared with the remaining colon cancer cell lines. Furthermore, overexpression Wnt6 resulting from transfection of pGPU6/GFP/Neo-Wnt6-Homo-1 plasmid promoted the proliferation, cell cycle and migration of HCT116 and SW480 cells, but inhibited cell apoptosis . The expression of caspase-3 and MMP2 was increased, whereas the expression of Bax was decreased in response to upregulation of Wnt6. These results suggested that Wnt6 may serve a vital function in the development of colon cancer.
据报道，Wnt蛋白有助于多种类型癌症的进展。Wnt6是Wnt家族的成员，可能促进胃肠道癌和宫颈癌的肿瘤发生。在本研究中，评估了Wnt6在人结肠癌细胞系中的表达，以研究Wnt6在结肠癌发生发展中的作用。此外，还研究了Wnt6上调或下调对结肠癌细胞增殖、凋亡、细胞周期和细胞迁移的影响。此外，采用蛋白质印迹分析来评估Wnt6、B细胞淋巴瘤2相关X蛋白（Bax）、半胱天冬酶-3和基质金属蛋白酶（MMP）2的表达。本研究结果表明，与其余结肠癌细胞系相比，HCT116和SW480细胞中Wnt6的表达增加。此外，转染pGPU6/GFP/Neo-Wnt6-Homo-1质粒导致的Wnt6过表达促进了HCT116和SW480细胞的增殖、细胞周期和迁移，但抑制了细胞凋亡。随着Wnt6上调，半胱天冬酶-3和MMP2的表达增加，而Bax的表达降低。这些结果表明，Wnt6可能在结肠癌的发生发展中起重要作用。
