Mackler B P, Herbert V
Am J Clin Nutr. 1985 Oct;42(4):618-28. doi: 10.1093/ajcn/42.4.618.
Iron ascorbate chelate was prepared and purified with iron and ascorbate combined in a 1:1 molar ratio. Some iron dissociated from the chelate and bound to raw wheat bran and alfalfa meal, indicating something in these fiber sources produced the dissociation. The chelate bound intact to alpha-cellulose in bicarbonate and phosphate buffer. More iron as ferric chloride bound to the three fiber sources than did iron as chelate. Iron became more soluble in bicarbonate buffer in the presence of raw wheat bran and alfalfa meal, but became less soluble in phosphate buffer in the presence of these fiber sources.