Yardimci Gozde Oren, Cekmecelioglu Deniz
Department of Food Engineering, Middle East Technical University, 06800 Ankara, Turkey.
3 Biotech. 2018 Jul;8(7):290. doi: 10.1007/s13205-018-1315-y. Epub 2018 Jun 25.
Co-cultures of and were investigated in submerged fermentation for xylanase production at shake-flask scale. Xylanase production markedly increased when arabinose, xylose, or hazelnut shells were used as the single carbon source. Maximal xylanase of 49.5 IU/mL was achieved with 4% , 4% , 40% solid load of hazelnut shells, and pH 7.0. Overall, xylanase was enhanced by 4.4-fold compared to initial un-optimized monoculture production and 2.8-fold compared to initial un-optimized co-cultured production, after optimization by response surface method.
在摇瓶规模的深层发酵中研究了[具体菌种1]和[具体菌种2]的共培养用于木聚糖酶生产。当使用阿拉伯糖、木糖或榛子壳作为单一碳源时,木聚糖酶产量显著增加。在4%[具体物质1]、4%[具体物质2]、40%榛子壳固体负载量和pH 7.0条件下,木聚糖酶的最大产量达到49.5 IU/mL。总体而言,通过响应面法优化后,木聚糖酶产量相较于初始未优化的单一培养产量提高了4.4倍,相较于初始未优化的共培养产量提高了2.8倍。