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Production of a human monoclonal antibody to HLA by human-human hybridoma technology. A preliminary report.用人-人杂交瘤技术制备抗人白细胞抗原的人单克隆抗体。初步报告。
Am J Pathol. 1985 Oct;121(1):10-4.
2
Establishment of hybridoma secreting human monoclonal antibody against hepatitis B virus surface antigen.分泌抗乙型肝炎病毒表面抗原人单克隆抗体杂交瘤的建立。
Biochem Biophys Res Commun. 1987 Feb 13;142(3):805-12. doi: 10.1016/0006-291x(87)91485-9.
3
Characterization of stable Epstein-Barr (EB) virus transformed cell lines and mouse-human hybridomas producing a large quantity of anti-tetanus toxoid (TT) monoclonal antibody.稳定的爱泼斯坦-巴尔（EB）病毒转化细胞系及产生大量抗破伤风类毒素（TT）单克隆抗体的小鼠-人杂交瘤的特性分析
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Human anti-tetanus toxoid monoclonal antibody secreted by EBV-transformed human B cells fused with murine myeloma.由EB病毒转化的人B细胞与鼠骨髓瘤细胞融合分泌的人抗破伤风类毒素单克隆抗体。
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The construction and use of a human-mouse myeloma analogue suitable for the routine production of hybridomas secreting human monoclonal antibodies.一种适用于常规生产分泌人单克隆抗体杂交瘤的人-鼠骨髓瘤类似物的构建与应用。
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Production of anti-tumor human monoclonal antibodies using different approaches.使用不同方法生产抗肿瘤人单克隆抗体。
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A human myeloma cell line suitable for the generation of human monoclonal antibodies.一种适合用于产生人单克隆抗体的人骨髓瘤细胞系。
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Human monoclonal antibody to purified protein derivative of tuberculin produced by hybrids constructed with Epstein-Barr virus-transformed B lymphocytes and mouse myeloma cells.由爱泼斯坦-巴尔病毒转化的B淋巴细胞与小鼠骨髓瘤细胞构建的杂交瘤产生的结核菌素纯蛋白衍生物的人单克隆抗体。
Eur J Immunol. 1986 May;16(5):584-7. doi: 10.1002/eji.1830160522.

引用本文的文献

1
Hybridoma technology: new developments of practical interest.杂交瘤技术：具有实际意义的新进展。
J Immunol Methods. 1987 Aug 3;101(2):153-70. doi: 10.1016/0022-1759(87)90147-5.

本文引用的文献

1
MICRODROPLET ASSAY OF HUMAN SERUM CYTOTOXINS.人血清细胞毒素的微滴分析法
Nature. 1964 Dec 5;204:998-1000. doi: 10.1038/204998b0.
2
Human monoclonal antibodies.
Nature. 1982 Nov 25;300(5890):316-7. doi: 10.1038/300316a0.
3
HLA monoclonal antibody registry: third listing.人类白细胞抗原单克隆抗体登记处：第三次清单
Tissue Antigens. 1984 Oct;24(4):209-14. doi: 10.1111/j.1399-0039.1984.tb02129.x.
4
A strategy for the production of human monoclonal antibodies reactive with lung tumor cell lines.一种生产与肺肿瘤细胞系发生反应的人单克隆抗体的策略。
Cancer Res. 1984 Jul;44(7):2750-3.
5
Human hybridomas constructed with antigen-specific Epstein-Barr virus-transformed cell lines.用人抗原特异性爱泼斯坦-巴尔病毒转化细胞系构建的人杂交瘤。
Proc Natl Acad Sci U S A. 1982 Nov;79(21):6651-5. doi: 10.1073/pnas.79.21.6651.
6
Monoclonal antibodies to HLA determinants.针对人类白细胞抗原（HLA）决定簇的单克隆抗体。
Br Med Bull. 1984 Jul;40(3):267-75. doi: 10.1093/oxfordjournals.bmb.a071988.
7
A rapid banding technique for human chromosomes.一种用于人类染色体的快速显带技术。
Lancet. 1971 Oct 30;2(7731):971-2. doi: 10.1016/s0140-6736(71)90287-x.
8
Detection of antibodies to cell surface antigens by a simplified cellular ELISA (CELISA).
Tissue Antigens. 1985 Apr;25(4):204-11. doi: 10.1111/j.1399-0039.1985.tb00438.x.
9
Continuous cultures of fused cells secreting antibody of predefined specificity.分泌预定义特异性抗体的融合细胞的连续培养物。
Nature. 1975 Aug 7;256(5517):495-7. doi: 10.1038/256495a0.

用人-人杂交瘤技术制备抗人白细胞抗原的人单克隆抗体。初步报告。

Production of a human monoclonal antibody to HLA by human-human hybridoma technology. A preliminary report.

作者信息

Hulette C M, Effros R B, Dillard L C, Walford R L

出版信息

Am J Pathol. 1985 Oct;121(1):10-4.

PMID:2996358
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1888038/
Abstract

An Epstein-Barr-virus-transformed lymphoblastoid cell line (ECEBV) was derived from a multiply transfused renal dialysis patient. ECEBV was shown to secrete specific antibody in a cellular enzyme-linked immunosorbent assay (CELISA) and was hybridized with the mutagenized human fusion partner G M1500 resistant to 6-thioguanine and ouabain. Hybridomas surviving hypoxanthine-aminopterin-thymidine (HAT) and ouabain selection were cloned by limiting dilution. The hybridomas continue to secrete antibody which reacts with some human cells but not with others after 14 months in culture. None reacts with K562 (no HLA-A, -B, -C or -DR) or with Daudi (no HLA-A, -B, or -C). This is a preliminary report of the production of a human monoclonal antibody to HLA. Application of this technique could result in the large-scale production of human monoclonal antibodies for HLA typing, the production of anti-idiotype antibodies for use in transplant patients to prevent acute rejection, and for the study of the structure and function of HLA in man.

摘要

一株爱泼斯坦-巴尔病毒转化的淋巴母细胞系（ECEBV）源自一名多次输血的肾透析患者。在细胞酶联免疫吸附测定（CELISA）中，ECEBV被证明能分泌特异性抗体，并与对6-硫鸟嘌呤和哇巴因耐药的诱变人融合伙伴GM1500杂交。通过有限稀释法对在次黄嘌呤-氨基蝶呤-胸腺嘧啶核苷（HAT）和哇巴因选择中存活的杂交瘤进行克隆。这些杂交瘤在培养14个月后仍继续分泌与一些人类细胞反应但不与其他细胞反应的抗体。没有一个与K562（无HLA-A、-B、-C或-DR）或Daudi（无HLA-A、-B或-C）反应。这是关于一种抗HLA人单克隆抗体产生的初步报告。应用该技术可大规模生产用于HLA分型的人单克隆抗体、用于移植患者预防急性排斥的抗独特型抗体，以及用于研究人类HLA的结构和功能。