Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, Laboratory of Evolutionary Theranostics, School of Biomedical Engineering, Health Science Center , Shenzhen University , Shenzhen 518060 , China.
Anal Chem. 2018 Aug 7;90(15):9381-9385. doi: 10.1021/acs.analchem.8b01992. Epub 2018 Jul 13.
Photoacoustic detection is an emerging noninvasive and nonionizing detection technique with the merits of rich contrast, high resolution, and deep tissue penetration, especially for in vivo detection and imaging. Herein, we developed a photoacoustic (PA) molecular imaging probe (denoted as nanonaps) composed of a naphthalocyanine dye and a heptamethine dye as the internal standard with unchanged signals at 860 nm and the sensing component with peroxynitrite (ONOO) target-decreased signals at 775 nm, respectively. The as-prepared nanonaps displayed high sensitivity and specificity of ONOO both in vitro and in vivo. The PA/PA ratio was increased as a function of the concentration of ONOO (0-250 nM). More interestingly, our ratiometric nanonaps could be used for in vivo detection and imaging of ONOO.
光声检测是一种新兴的非侵入式和非电离检测技术，具有丰富的对比度、高分辨率和深组织穿透的优点，特别是在体内检测和成像方面。在这里，我们开发了一种光声（PA）分子成像探针（表示为 nanonaps），由萘酞菁染料和七甲川染料组成，作为内部标准，在 860nm 处的信号不变，而作为传感组件的过氧亚硝酸盐（ONOO）目标的信号在 775nm 处降低。所制备的 nanonaps 在体外和体内均表现出对 ONOO 的高灵敏度和特异性。PA/PA 比随着 ONOO 浓度（0-250 nM）的增加而增加。更有趣的是，我们的比率纳米颗粒可用于体内检测和成像的 ONOO。
