Kuzuya F, Naito M, Asai K I, Shibata K
Angiology. 1985 Sep;36(9):622-7. doi: 10.1177/000331978503600906.
When cultured with a Ca2+ antagonist diltiazem, fetal bovine aortic smooth muscle cells showed a cobblestone-like monolayer and a positive factor VIII-related antigen in culture, which are supposed to be specific to the endothelial cells in general. Similar change was produced by addition of a cyclic AMP analogue, dibutyryl cyclic AMP or a phosphodiesterase inhibitor, theophylline. All of these changes were reversible. Both smooth muscle cells and endothelial cells are derived from mesenchymal cells embryologically and it is of great interest to presume the differentiation process of both cells.
当与钙离子拮抗剂地尔硫卓一起培养时，胎牛主动脉平滑肌细胞在培养物中呈现出鹅卵石样的单层结构以及与因子VIII相关的抗原阳性，而这些通常被认为是内皮细胞所特有的。添加环磷酸腺苷类似物二丁酰环磷酸腺苷或磷酸二酯酶抑制剂茶碱也会产生类似的变化。所有这些变化都是可逆的。平滑肌细胞和内皮细胞在胚胎学上均源自间充质细胞，推测这两种细胞的分化过程非常有趣。
