Kuzuya F, Naito M, Asai K, Shibata K, Iwata Y
Artery. 1983;12(1):51-9.
Smooth muscle cells harvested from fetal bovine aortic media were grown in subculture in Waymouth medium containing 10% fetal bovine serum for 14 days. Lanthanum (La3+), an inorganic Ca2+- blocker, was added in a concentration of 10(-3) M to the medium. The cultured smooth muscle cells showed short-spindle-shaped or polygonal endothelial cell-like forms, keeping a monolayer of paving-stone pattern and reached the stationary phase. They stayed in a monolayer state until the end of the 14-day culture. By fluorescent staining with FITC-labelled anti-factor VIII serum, the presence of factor VIII antigen, which is regarded as characteristic of endothelial cells in general, was demonstrated. When the medium was changed to the medium not containing La3+, the usual state of smooth muscle cells showing spindle-shaped multilayer proliferation was recovered, therefore demonstrating that this change is reversible.
从胎牛主动脉中膜收获的平滑肌细胞在含有10%胎牛血清的Waymouth培养基中传代培养14天。无机钙离子阻滞剂镧（La3+）以10（-3）M的浓度添加到培养基中。培养的平滑肌细胞呈现短梭形或多边形内皮细胞样形态，保持单层铺路石样模式并达到静止期。它们在14天培养结束前一直处于单层状态。通过用异硫氰酸荧光素（FITC）标记的抗因子VIII血清进行荧光染色，证实了因子VIII抗原的存在，一般认为该抗原是内皮细胞的特征。当培养基更换为不含La3+的培养基时，平滑肌细胞恢复了通常的梭形多层增殖状态，因此表明这种变化是可逆的。
