Goncu Beyza, Yucesan Emrah, Ozdemir Burcu, Basoglu Harun, Kandas Nur Ozten, Akbas Fahri, Aysan Erhan
1 Experimental Research Center, Bezmialem Vakif University , Istanbul, Turkey .
2 Institute of Life Sciences and Biotechnology, Bezmialem Vakif University , Istanbul, Turkey .
Biopreserv Biobank. 2018 Aug;16(4):278-284. doi: 10.1089/bio.2018.0008. Epub 2018 Jul 2.
Cold ischemia protects organs and tissues by slowing their metabolism, but it also causes ischemic injury. Minimizing cold ischemia has been an important goal in parathyroid auto- and allotransplantation, as well as the transplantation of other major organs. Parathyroid glands are responsible for calcium homeostasis by releasing parathormone (PTH) into the blood circulation. Functionality of a new parathyroid transport solution (NPTS) and effects on cell viability, PTH secretion, and calcium-sensing receptor (CaSR) levels during cold ischemia were evaluated.
A NPTS was prepared, and the pH was adjusted to a range of 7.2-7.4 and kept at +4°C until use. Seven patients with parathyroid hyperplasia secondary to chronic renal failure who were scheduled to undergo subtotal parathyroidectomy were enrolled in the study. Glands were cold-preserved in NPTS with different time intervals (0, 6, 12, 18, and 24 hours), and then parathyroid cell viability before and after cryopreservation, PTH secretion, and CaSR levels were determined.
The mean cell viability before cryopreservation was 92.7% (range 89.2%-97.2%). There were no significant differences in cell viability rates before and after cryopreservation (p = 0.1168 and p = 0.4085, respectively), and CaSR levels (p = 0.5446) were not significant.
NPTS is a solution designed specifically for parathyroid tissue transplantation. This patent pending product can support cellular viability and PTH release, as well as protect CaSR functionality for up to 24 hours of cold ischemia.
冷缺血通过减缓器官和组织的新陈代谢来保护它们，但也会导致缺血性损伤。尽量减少冷缺血时间一直是甲状旁腺自体移植和同种异体移植以及其他主要器官移植的重要目标。甲状旁腺通过将甲状旁腺激素（PTH）释放到血液循环中来维持钙稳态。评估了一种新型甲状旁腺转运溶液（NPTS）的功能以及在冷缺血期间对细胞活力、PTH分泌和钙敏感受体（CaSR）水平的影响。
制备NPTS，将pH值调节至7.2 - 7.4范围，并保持在+4°C直至使用。纳入7例计划行甲状旁腺次全切除术的慢性肾衰竭继发性甲状旁腺增生患者。腺体在NPTS中进行不同时间间隔（0、6、12、18和24小时）的冷保存，然后测定冷冻保存前后的甲状旁腺细胞活力、PTH分泌和CaSR水平。
冷冻保存前的平均细胞活力为92.7%（范围89.2% - 97.2%）。冷冻保存前后的细胞活力率无显著差异（分别为p = 0.1168和p = 0.4085），CaSR水平（p = 0.5446）也无显著差异。
NPTS是一种专门为甲状旁腺组织移植设计的溶液。这种正在申请专利的产品可以支持细胞活力和PTH释放，并在长达24小时的冷缺血期间保护CaSR功能。
