Aiti Annalisa, Rossi Martina, Alviano Francesco, Morara Barbara, Burgio Luca, Cioccoloni Eleonora, Cavicchi Ottavio, Pasquinelli Gianandrea, Bonsi Laura, Buzzi Marina
Emilia Romagna Cord Blood Bank, Immunohaematology and Transfusion Medicine, St. Orsola-Malpighi University Hospital, Bologna, Italy.
Unit of Histology, Embryology and Applied Biology, Department of Experimental, Diagnostic and Specialty Medicine, University of Bologna, Bologna, Italy.
Biopreserv Biobank. 2019 Oct;17(5):418-424. doi: 10.1089/bio.2018.0140. Epub 2019 Apr 25.
Parathyroidectomy is a standard practice to treat recurrent or persistent hyperparathyroidism. However, this can lead to the onset of hypoparathyroidism, treatable with the autotransplantation of parathyroid tissue (PT). Tissue can be transplanted immediately after parathyroidectomy or cryopreserved and transplanted only in case of necessity. Since 2011, the Cord Blood Bank and Cardiovascular Tissue Bank of Emilia-Romagna has been storing PT for potential autologous transplantation. To date, there are highly variable data about the viability and function of PT after thawing. However, it is not clear if the PT quality is affected by different cryopreservation protocols and/or by the storage time. The aim of this study was to assess the function and viability of the PTs of ten patients stored in the Bank. Tissue morphology was evaluated before and after cryopreservation through histological investigations. PT function was analyzed by assessing the ability of cryopreserved PT to synthesize and secrete parathyroid hormone (PTH) in response to different calcium concentrations. Moreover, viability and function were also investigated on tissue-isolated cells in culture. These data show that tested tissues appear to be viable and able to produce PTH even after 5 years of storage, and the histological architecture is well preserved.
甲状旁腺切除术是治疗复发性或持续性甲状旁腺功能亢进的标准方法。然而，这可能导致甲状旁腺功能减退的发生，可通过甲状旁腺组织（PT）自体移植进行治疗。组织可在甲状旁腺切除术后立即移植，也可冷冻保存，仅在必要时移植。自2011年以来，艾米利亚-罗马涅脐带血库和心血管组织库一直在储存PT用于潜在的自体移植。迄今为止，关于解冻后PT的活力和功能的数据差异很大。然而，尚不清楚PT质量是否受不同冷冻保存方案和/或储存时间的影响。本研究的目的是评估储存在该库中的10例患者的PT的功能和活力。通过组织学研究评估冷冻保存前后的组织形态。通过评估冷冻保存的PT在不同钙浓度下合成和分泌甲状旁腺激素（PTH）的能力来分析PT功能。此外，还对培养的组织分离细胞的活力和功能进行了研究。这些数据表明，即使在储存5年后，测试的组织似乎仍具有活力且能够产生PTH，并且组织结构保存良好。
