Saijuntha Weerachai, Tantrawatpan Chairat, Agatsuma Takeshi, Wang Chennan, Intapan Pewpan M, Maleewong Wanchai, Petney Trevor N
Walai Rukhavej Botanical Research Institute (WRBRI), Biodiversity and Conservation Research Unit, Mahasarakham University, Maha Sarakham, 44150, Thailand.
Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani, 12120, Thailand.
Mol Biochem Parasitol. 2018 Jul;223:31-36. doi: 10.1016/j.molbiopara.2018.06.004. Epub 2018 Jun 28.
Fasciola hepatica and F. gigantica are ruminant liver flukes that are found worldwide. They can occur sympatrically and hybridize, consequently producing an intermediate form (Fasciola sp.), especially in Africa and Asia. The bridge intron (TkBridgeInt) and intron 4 of domain 2 (TkD2Int4) of the taurocyamine kinase gene were cloned and sequenced from 14 F. hepatica, 18 F. gigantica, and 12 Fasciola sp. These intron regions could be used to differentiate F. hepatica from F. gigantica and revealed genetic hybridization and DNA recombination between F. hepatica and F. gigantica in Fasciola sp. from Japan and Bangladesh. Two specimens of F. hepatica from Ecuador revealed cryptic diversity. A combination of three haplotypes observed in a Fasciola sp. specimen from Japan suggested triploidy. Thus, the intron region of the taurocyamine kinase gene is a potential marker for detecting the hybrid status of the Fasciola flukes.
肝片吸虫和巨片吸虫是在全球范围内均有发现的反刍动物肝脏吸虫。它们可以同域分布并杂交，从而产生一种中间形态（片形吸虫属），尤其是在非洲和亚洲。从14条肝片吸虫、18条巨片吸虫和12条片形吸虫属中克隆并测序了牛磺酸胺激酶基因的桥接内含子（TkBridgeInt）和结构域2的内含子4（TkD2Int4）。这些内含子区域可用于区分肝片吸虫和巨片吸虫，并揭示了来自日本和孟加拉国的片形吸虫属中肝片吸虫和巨片吸虫之间的遗传杂交和DNA重组。来自厄瓜多尔的两条肝片吸虫标本显示出隐秘的多样性。在来自日本的一个片形吸虫属标本中观察到的三种单倍型组合表明存在三倍体现象。因此，牛磺酸胺激酶基因的内含子区域是检测片形吸虫杂交状态的潜在标记。
